Summary of Study ST001802
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001136. The data can be accessed directly via it's Project DOI: 10.21228/M8VQ4D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001802 |
Study Title | Human lung exposomics analysis |
Study Type | Untargeted MS anlaysis |
Study Summary | We tested the general utility of XLE in a variety of human biological samples by analyzing human lung and thyroid tissues and stool samples. We quantified 32 environmental chemicals in 11 human lungs, with HCB, PCB-28 and PCB-18 being most frequently detected (10 out of 11). The commonly detected chemicals in human plasma were detected less frequently in the lung. For the 11 lungs, p,p’-DDE was detected in eight, PCB-153 in five, PBDE-47 and PCB-138 in four and PCB-180 in three. Although the plasma samples were from non-diseased individuals and the lungs were both diseased and non-diseased individuals, HCA results suggest that environmental chemical profiles in human lung may be very different from plasma. |
Institute | Emory University |
Department | Medicine/Pulmonary |
Laboratory | Dean Jones |
Last Name | Hu |
First Name | Xin |
Address | Emory University Whitehead building (Rm 225), 615 Michael Street |
xin.hu2@emory.edu | |
Phone | 4047275091 |
Submit Date | 2021-05-06 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | GC-MS |
Release Date | 2021-05-28 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001892 |
Treatment Summary: | Tissue materials were processed similarly with a consistent ratio of 1:5 (sample to hexane-ethyl acetate mixture), i.e., 100 mg lung was homogenized in 300 µL water and extracted with 150 µL formic acid and 400 µL hexane-ethyl acetate mixture, while 40 mg thyroid was homogenized in 250 µL water and extracted with 50 µL formic acid and 200 µL hexane-ethyl acetate mixture. Stool samples (100 mg) were homogenized and extracted directly in 50 µL formic acid and 500 µL hexane-ethyl acetate mixture and then processed as plasma samples. The variation of 1:4 from 1:5 in lung extraction was arbitrary in consideration of the lower density of lung as an organ. The internal standards were spiked at final concentration: 1 ng/mL. The sample mixture was shaken vigorously on ice using multi-tube vortexer (VWR VX-2500) for 1 h and centrifuged at 1000 g, 4 °C for 10 min. The sample mixture was chilled during entire extraction procedure. The organic supernatant was transferred to a new tube with 25 mg MgSO4 (≥99.99% pure, Sigma-Aldrich) for testing of QuEChERS based procedure, and vortexed vigorously to remove water. After 10 min centrifugation at 1000 g, 80 µL of the final supernatant was spiked with instrumental internal standards (final concentration: 1 ng/mL) for analysis. |