Summary of Study ST001962
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001249. The data can be accessed directly via it's Project DOI: 10.21228/M88974 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001962 |
| Study Title | Nontargeted metabolomics of UGT71 CRISPR knockouts in Poplar |
| Study Summary | Non-targeted metabolomic analysis of UGT71L1 CRISPR-Cas9 knockouts in populus tremula x populus alba |
| Institute | University of Victoria |
| Last Name | Gordon |
| First Name | Harley |
| Address | 3800 Finnerty Rd |
| harleygordon@uvic.ca | |
| Phone | 2507217211 |
| Submit Date | 2021-10-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-12-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR002054 |
| Treatment Summary: | Leaf tissue from Populus tremula x Populus alba was transformed using CRISPR-Cas9 to create functional knockouts of the UGT71L1 gene. Positive transformants were selected with Kanamycin and sequencing was conducted to confirm transformation. Clones of either wild type plants, knockouts (frame shift mutations, both alleles), intermediates (Frameshift mutation, one allele), or empty vectors (no gRNA for Cas9 construct). Plants were then grown under identical conditions for three months in a greenhouse. |
| Treatment: | Genetic |
| Plant Growth Location: | Greenhouse |
| Plant Watering Regime: | As required |
