Summary of Study ST002207
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001410. The data can be accessed directly via it's Project DOI: 10.21228/M8G42C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002207 |
| Study Title | Metabolomic analysis to assess response to immunotherapy for malignant brain tumors: Part 1 |
| Study Type | Cancer surrogate biomarker discovery |
| Study Summary | An effective immune response in patients with cancer treated with immunotherapy includes dendritic cell (DC) activation and migration followed by stimulation of CD8 and CD4 T cells. This then leads to the activation, proliferation and further activation of other immune cell populations including NK cells or immunosuppressive populations such as Tregs and myeloid derived suppressor cells (MDSCs). These studies were carried out utilizing murine brain tumor models treated with an RNA DC vaccine platform. We hypothesized that metabolomic analyses of urines would be sensitive to the action of this diverse set of immune cells. The objective of this study was to evaluate the feasibility of using metabolomics to follow immune responses after immunotherapy. We chose NMR as our analytical technique of choice, as it has many favorable qualities that make it ideal for analyses of urine. |
| Institute | University of Florida |
| Department | Neurosurgery |
| Laboratory | Rm 042 |
| Last Name | Khattri |
| First Name | Ram |
| Address | 1864 Stadium RD, |
| rbk11@ufl.edu | |
| Phone | 330-785-6045 |
| Submit Date | 2022-06-09 |
| Num Groups | 3 |
| Total Subjects | 15 |
| Num Males | NA |
| Num Females | NA |
| Study Comments | Metabolomic profiling of urine samples |
| Publications | Metabolomics journal (submitted) |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-06-16 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002305 |
| Treatment Summary: | Dendritic cell vaccine and anti-PD-1 immunotherapy. Bone marrow (BM) was harvested from the long bones and sternum of euthanized animals and the red blood cells (RBCs) were lysed. Then myeloid-derived cells were cultured in DC complete media including granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin 4. Cells were cultured in six well plates for six days. On day seven, cells were re-plated in 60mm dishes. DCs were then electroporated with OVA-mRNA at day eight. On day nine, DCs were collected in phosphate buffered saline (PBS) for administration. DC vaccines were delivered once via intradermal injection in the inguinal area. Naïve C57/BL6 mice received antigen-specific T cells through intravenous (IV) and the DC vaccine injection intradermally on the same day. Both antigen-specific T cells and DC vaccine were administered once. Urine or serum samples were collected after DC vaccination at several timepoints. Finally, animals were euthanized when they reached the endpoints. |
| Animal Anesthesia: | isoflurane |
| Animal Fasting: | non-fasted |
| Animal Endp Euthanasia: | Euthanasia was carried out by thoracotomy followed by cervical dislocation. |
