Summary of Study ST002848
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001783. The data can be accessed directly via it's Project DOI: 10.21228/M87M7X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002848 |
| Study Title | Lipidomics for linked CD1 proteins |
| Study Summary | In this study, the four types of human CD1 antigen-presenting molecules were constructed using a protein linker between heavy chains of CD1 proteins and beta 2 macroglobulin (B2m), thus called linked CD1 proteins, expressed in human myelogenous cell line K562 cell, purified using affinity chromatography, and extracted using the adapted Folch method for bound lipids. These extracted lipids in triplicates from four CD1 proteins were profiled using the extraction from in parallel generated HLA-B27 protein as a control. Data demonstrated differing lipid capture motifs based on the length and chemical structures of lipids bound, pointing to general self-lipid capture mechanisms. For CD1a and CD1d, lipid size matches CD1 cleft volume, whereas CD1b shows a nearly universal size mismatch with its ligands, which results from the uniform seating of two small lipids within its large cleft. Further, the list of compounds that comprise the assembled CD1 lipidomes provides a resource for matching to bioactive lipids from other fields of research and supports the ongoing discovery of lipid blockers and antigens for T cells. |
| Institute | Brigham and Women's Hospital |
| Last Name | Huang |
| First Name | Shouxiong |
| Address | 251 Kettering Laboratory Complex, 160 Panzeca Way, Cincinnati, OH |
| shouxiong.huang@uc.edu | |
| Phone | 5135587572 |
| Submit Date | 2023-08-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzdata.xml |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-10 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002969 |
| Treatment Summary: | Lipidomic profiles were generated from lipids that were extracted from K562 expressed CD1a, CD1b, CD1c, and CD1d proteins compared with peptide-binding protein HLA-B27 and buffer-only controls. No other relevant treatment. |
