Summary of Study ST002932

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001823. The data can be accessed directly via it's Project DOI: 10.21228/M82Q61 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002932
Study Title	Analyzing Metabolic Alterations in the Gut, Hippocampus and Brainstem of Mice Induced by Running Exercise Through Liquid Chromatography Mass Spectrometry
Study Summary	Metabolic analysis of the impact of running exercise on the gut, hippocampus and brainstem is crucial as it provides insights into how exercise affects memory, mood, and vital physiological functions. In our six-week study, we utilized a mouse model (C57BL/6 genotype) to investigate these metabolic changes. Employing liquid chromatography coupled with mass spectrometry followed by metabolomics for a comprehensive analysis, our approach offers insights into how exercise influences metabolic processes, including brain function. Our findings hold the potential to shape more effective exercise strategies for enhancing overall health and cognitive function.
Institute	University of Puerto Rico, School of Medicine
Department	Biochemistry
Last Name	Chorna
First Name	Nataliya
Address	University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00935
Email	nataliya.chorna@upr.edu
Phone	7877582525 ext 1640
Submit Date	2023-10-15
Num Groups	2
Total Subjects	24
Num Males	24
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-10-30
Release Version	1

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Treatment:

Treatment ID:	TR003054
Treatment Summary:	To evaluate our hypothesis that running exercise reshapes gut microbiota diversity that balances TRP metabolism in the gut, we used an established model of voluntary running exercise in 20-week-old male mice (C57BL/6J, Jackson Lab) housed individually in a standard cage in temperature-controlled (21°C) quarters with a 12-h light/12-h dark cycle. Animals were given water and food (Purina Chow) ad libitum as previously described (Chorna et al., 2013). Briefly, mice were randomized into two groups, sedentary control (n=12) and running experimental (n=12), housed with free access to a wireless running wheel (Med Associates) for six weeks. For the sedentary group, the wheels were locked in the experiment. Therefore, this group of mice could not perform running exercises. Exercise activities of the running group were recorded for each animal for the investigation to ensure that each mouse was physically active. The recording was conducted using an automatic counter and Med Associates software.

Treatment ID:

TR003054

Treatment Summary:

To evaluate our hypothesis that running exercise reshapes gut microbiota diversity that balances TRP metabolism in the gut, we used an established model of voluntary running exercise in 20-week-old male mice (C57BL/6J, Jackson Lab) housed individually in a standard cage in temperature-controlled (21°C) quarters with a 12-h light/12-h dark cycle. Animals were given water and food (Purina Chow) ad libitum as previously described (Chorna et al., 2013). Briefly, mice were randomized into two groups, sedentary control (n=12) and running experimental (n=12), housed with free access to a wireless running wheel (Med Associates) for six weeks. For the sedentary group, the wheels were locked in the experiment. Therefore, this group of mice could not perform running exercises. Exercise activities of the running group were recorded for each animal for the investigation to ensure that each mouse was physically active. The recording was conducted using an automatic counter and Med Associates software.