Summary of Study ST003067
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001913. The data can be accessed directly via it's Project DOI: 10.21228/M8FH9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003067 |
| Study Title | Attenuation of Helicobacter pylori VacA toxin-induced cell death by modulation of intracellular taurine metabolism - Study #1 |
| Study Type | untargeted metabolomics analysis |
| Study Summary | VacA alters the metabolite content of AGS cells. To detect potential VacA-induced metabolic alterations in host cells, we treated AGS cells with acid-activated VacA (20 μg/ml) or acidified buffer control for 2, 8, or 12 hours and performed untargeted global metabolic on the harvested cell pellets. AGS cells treated with VacA under these conditions do not undergo VacA-induced cell death. Analysis of the results showed that AGS cells treated with VacA had a significantly different metabolic profile than cells treated with an acidified-buffer control, both for RPLC and HILIC analyses. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | CODREANU |
| First Name | SIMONA |
| Address | 1234 STEVENSON CENTER LANE |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6158758422 |
| Submit Date | 2024-02-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR003191 |
| Treatment Summary: | AGS and AZ-521 cells were cultured in T-75 cell culture flasks overnight to a density of approximately 4x106 cells. Cells were incubated in media containing 20 ug/mL of purified s1m1 VacA toxin and 5 mM ammonium chloride. Following intoxication, the media was removed, and cells were washed with PBS. Cells were detached by incubation with trypsin for 5 minutes and collected via centrifugation at 4°C at 1,000 rpm for 4 minutes. Trypsin was removed, cells were once again washed with PBS, and the cells were then flash-frozen in liquid nitrogen and stored at -70°C. |
| Treatment Protocol Filename: | Cell_Culture_Methods.pdf |
