Summary of Study ST003070
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001913. The data can be accessed directly via it's Project DOI: 10.21228/M8FH9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003070 |
| Study Title | Attenuation of Helicobacter pylori VacA toxin-induced cell death by modulation of intracellular taurine metabolism - Study #3 |
| Study Type | untargeted metabolomics analysis |
| Study Summary | Effects of VacA on metabolic profiles of AZ-521 cells. Previous studies showed that VacA causes vacuolation of both AGS and AZ-521 gastro-duodenal epithelial cell types. Notably, VacA treatment of AZ-521 cells with purified VacA for 24 hours results in cell death, whereas AGS cells are relatively resistant to VacA-induced cell death at this time point. To investigate potential similarities and differences in VacA-induced metabolic alterations in AGS and AZ-521 cells, we treated both AZ-521 cells and AGS cells with an acidified buffer control or WT s1m1 VacA (20 ug/mL) for 3 or 5 hours. VacA did not impact cell viability of either cell type at these timepoints. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | CODREANU |
| First Name | SIMONA |
| Address | 1234 STEVENSON CENTER LANE |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6158758422 |
| Submit Date | 2024-02-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR003194 |
| Treatment Summary: | AGS and AZ-521 cells were cultured in T-75 cell culture flasks overnight to a density of approximately 4x106 cells. Cells were incubated in media containing 20 ug/mL of purified s1m1 VacA toxin and 5 mM ammonium chloride. Following intoxication, the media was removed, and cells were washed with PBS. Cells were detached by incubation with trypsin for 5 minutes and collected via centrifugation at 4°C at 1,000 rpm for 4 minutes. Trypsin was removed, cells were once again washed with PBS, and the cells were then flash-frozen in liquid nitrogen and stored at -70C. |
| Treatment Protocol Filename: | MS_Methods_Cover_VacA_AGS_AZ521.pdf |
