Summary of project PR000125

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.


Project ID: PR000125
Project DOI:doi: 10.21228/M8DW2B
Project Title:Mammalian Suspension-Cultured Cellular Metabolomics Workflow
Project Type:Metabolomics, Lipidomics, Untargeted
Project Summary:A workflow was optimized for the sample preparation of a single suspension-cultured cell pellet for both metabolomics and lipidomics analysis. Jurkat T-lymphocyte cells were washed with various rinsing solutions and the lipids extracted using different lipid extraction protocols to allow for the most reproducible and quantitative method.
Institute:University of Florida
Department:Dept. of Chemistry/SECIM
Laboratory:Biomedical Mass Spectrometry Lab
Last Name:Yost
First Name:Richard
Address:P.O. Box 117200, Gainesville, FL 32611
Phone:(352) 392-0515
Funding Source:JDRF Research Grant

Summary of all studies in project PR000125

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST000140 Cell Rinsing Solution Comparison Homo sapiens University of Florida MS 2016-03-03 1 5 Uploaded data (3G)*
ST000246 Lipid Extraction Efficiency Comparison Homo sapiens University of Florida MS 2016-12-22 1 4 Uploaded data (2.1G)*