Summary of project PR000194
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000194. The data can be accessed directly via it's Project DOI: 10.21228/M89W2D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000194 |
| Project DOI: | doi: 10.21228/M89W2D |
| Project Title: | Cyclobutene- and cyclobutane-functionalized fatty acids as novel biochemical probes of structure and function in HepG2 cells |
| Project Type: | Lipidomics |
| Project Summary: | Five analogues of OA (18:1cis9) or elaidic acid (18:1trans9) replacing the alkene with a four-membered carbocycle were evaluated in HepG2 cells, which are well-characterized models for hepatocytes. In order to assess whether or not the novel analogues were incorporated into complex lipids, cells were treated with compond and then lipids extracted. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS) was employed for the analysis of complex lipids. Data processing involved mass detection, chromatographic peak detection and deconvolution, isotopic peaks grouping, normalization and peak alignment. Significantly altered metabolites were defined by a fold change (FC) >2 and p<0.05. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) of signature metabolites altered in compounds treated cells compared to control were performed in the Metaboanalyst web portal (www.metaboanalyst.ca). |
| Institute: | University of Nebraska-Lincoln |
| Department: | Biochemistry |
| Laboratory: | DiRusso Black FATTT Lab |
| Last Name: | DiRusso |
| First Name: | Concetta |
| Address: | Department of Biochemistry, University of Nebraska-Lincoln, N241 Beadle Center 1901 Vine St. |
| Email: | cdirusso2@unl.edu |
| Phone: | 402-472-6504 or 402-613-9293 |
| Funding Source: | NIH |
Summary of all studies in project PR000194
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000241 | Cyclobutene- and cyclobutane-functionalized fatty acids as novel biochemical probes of structure and function in HepG2 cells | Homo sapiens | University of Nebraska-Lincoln | MS | 2017-07-10 | 1 | 24 | Uploaded data (4.8G)* |
