Summary of project PR000285
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000285. The data can be accessed directly via it's Project DOI: 10.21228/M82W27 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000285 |
| Project DOI: | doi: 10.21228/M82W27 |
| Project Title: | Metabolic Profiling of Anxiety Prone HSV-Latently Infected Obese Mice |
| Project Type: | Broad Spectrum LCMS |
| Project Summary: | The biological factors that lead children from low socioeconomic backgrounds to be at greater risk for the development of anxiety and learning problems are not well understood. While it is clear that there are genetic components to the risk of developing mental health disorders, a role for environmental factors in inducing these problems has been suggested. Many of these factors that affect the brain likely involve exposures that occur early in life. Two such factors are the higher rates of herpes simplex virus (HSV)-1 seropositivity and prevalence of obesity among these children. HSV-1 infection has been associated with impaired cognition during childhood and mental health problems in adulthood. Additionally, obese adults are shown to have higher HSV-1 titers. Similarly, other studies have correlated a diet high in saturated fat and obesity with increased risk of mood disorders and anxiety. A mouse model of obese HSV-1 latent infection was developed. Broad spectrum metabolomics analysis was performed to better understand the metabolomic profile of hippocampus and to compare this metabolomics profile with that of the hypothalamus, microglia, and peripheral blood mononuclear cells. Brain tissue samples for metabolomics experiments were generated in the following manner: 3-week old mice were placed on a 10% low fat (LF) diet and acclimated for one week prior to intranasal HSV-1 infection or mock infection with PBS. Fourteen days post-infection mice were randomized to either a 45% high fat diet (HF) or remained on the LF diet. Eight weeks post-diet transition, mice were euthanized and brain tissue samples were collected and processed for metabolomics. |
| Institute: | University of North Carolina at Chapel Hill |
| Department: | Gillings School of Public Health |
| Laboratory: | Department of Nutrition |
| Last Name: | Sheridan |
| First Name: | Patricia |
| Address: | 2002 Hooker Research Center, CB#7461, Chapel Hill NC, 27599 |
| Email: | patricia_sheridan@med.unc.edu |
| Phone: | 919-843-6434 |
| Funding Source: | NIH, Common Fund, Pilot and Feasibility |
Summary of all studies in project PR000285
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000357 | Broad Spectrum MS analysis of mouse hypothalmus from Anxiety Prone HSV-Latently Infected Obese Mice | Mus musculus | University of North Carolina | MS* | 2017-03-03 | 1 | 36 | Uploaded data (32.7G)* |
| ST000358 | Broad Spectrum MS analysis of mouse hippocampus from Anxiety Prone HSV-Latently Infected Obese Mice | Mus musculus | RTI International | MS* | 2017-03-04 | 1 | 36 | Uploaded data (35.6G)* |
| ST000359 | Broad Spectrum MS analysis of mouse microglia cells from Anxiety Prone HSV-Latently Infected Obese Mice | Mus musculus | University of North Carolina | MS* | 2017-03-03 | 1 | 35 | Uploaded data (29.1G)* |
| ST000363 | Broad Spectrum MS analysis of mouse peripheral blood mononuclear cells (PBMC) from Anxiety Prone HSV-Latently Infected Obese Mice | Mus musculus | University of North Carolina | MS* | 2017-03-03 | 1 | 35 | Uploaded data (36.9G)* |
