Summary of project PR000430
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000430. The data can be accessed directly via it's Project DOI: 10.21228/M8GC82 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000430 |
| Project DOI: | doi: 10.21228/M8GC82 |
| Project Title: | Mayo Metabolomics Pilot and Feasibility Award: Metabolomics of Exhaled Breath Condensate in Decompensated Heart Failure |
| Project Summary: | Metabolomics is a large-scale approach to monitoring as many as possible of the compounds involved in cellular processes in a single assay to derive metabolic profiles. Compared with genomics or proteomics, metabolomics reflects changes in phenotype and therefore function. Nearly all the blood volume-cardiac output in humans flows through the lungs at a rate of 5L/min and traverses through capillaries with a very thin blood-gas barrier (approximately 2μm, 600–800 nm; in some places <200 nm) to allow sufficient gas diffusion. However, there is evidence that many other biochemicals from the blood can be found in the exhaled air. Collection of exhaled breath condensate (EBC) is a relatively new, noninvasive method that allows assessment of biochemical profiles that may not only link to airway physiology, but also to other changing processes in the body. The aims of this study are: (1) Working with the Mayo Clinic Metabolomics Resource Core Laboratory to develop the optimal method for performing metabolomics of EBC, (2) Collecting and metabolomic profiling of the EBCs from patients with heart failure; (2) Comparing the profiles of these patients obtained during stable and acute exacerbation periods as well as during recovery from a decompensation; (3) Comparing the profiles of these patients with age and gender matched controls; (4) Correlation of EBC metabolomic profiles with clinical phenotypes. The proposed metabolomics profiles would be obtained from patients involved in IRB approved, ongoing, primarily NIH funded studies focused on understanding pulmonary congestion in heart failure patients. This includes a recently started project capturing patients as they present in the emergency room with decompensated heart failure. Phenotyping for this study includes novel measures of pulmonary capillary recruitment, alveolar-capillary conductance, pulmonary capillary blood volume, thoracic CT imaging combined with in-hospital clinical data, including blood biomarkers such as BNP and catecholamines. Measurements are performed upon admission (within 24hours) and then repeated just prior to dismissal. In addition, we would collect EBC on stable HF patients and in age and gender matched controls healthy controls from our other ongoing studies. The overall aim will be to identify novel patterns or biomarkers for developing a point-of-care test (POC) for tracking disease status in HF patients. |
| Institute: | Mayo Clinic |
| Department: | Cardiovascular Diseases |
| Laboratory: | Mayo Metabolomics Core |
| Last Name: | Johnson |
| First Name: | Bruce |
| Address: | 200 First St. SW, Rochester, Minnesota, 55905, USA |
| Email: | johnson.bruce@mayo.edu |
| Phone: | 507-284-4441 |
Summary of all studies in project PR000430
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000587 | Metabolomics of Exhaled Breath Condensate in Decompensated Heart Failure | Homo sapiens | Mayo Clinic | NMR | 2019-05-15 | 1 | 23 | Not available |
| ST000588 | Metabolomics of Saliva in Decompensated Heart Failure | Homo sapiens | Mayo Clinic | NMR | 2019-05-15 | 1 | 24 | Not available |
