Summary of project PR000454
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000454. The data can be accessed directly via it's Project DOI: 10.21228/M8CC9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000454 |
| Project DOI: | doi: 10.21228/M8CC9H |
| Project Title: | Untargeted metabolomic changes in Chlamydomonas reinhardtii treated with lipid inducing small molecules |
| Project Summary: | A study to investigate the effect of small molecule lipid inducing compounds that leads to hyper accumulation of lipids in N replete cells of Chlamydomonas reinhardtii. These compounds were identified through a high throughput screening designed for that purpose. During that screening, we screened 43,783 compounds and identified 367 primary hits. These 367 hits were further retested using a 8-point dilution series (from 0.25 to 30 uM) and verified the activity of 250 compounds that induce the hyper lipid accumulating phenotype in algae. Once the hit compounds were identified and confirmed, we then performed extensive chemoinformatics analysis to look for common scaffolds and identified several common substructures. We then selected 15 top performing compounds from 5 diverse structural groups and tested biochemical parameters such as growth, lipid accumulating capacity, effect on photosynthetic rates, respiration rates, oxygen consumption rates, analysis of different lipid species to quantify and identify fatty acid species using GC-MS. To understand the global changes in the metabolome, 2 structurally different compounds were selected and compared with cells grown without compounds as control for untargeted metabolomics analysis. |
| Institute: | University of Nebraska-Lincoln |
| Department: | Biochemistry |
| Laboratory: | FATTTLab |
| Last Name: | Wase |
| First Name: | Nishikant |
| Address: | 1901 Beadle Center, Vine Street, 1901 VINE STREET, Lincoln, NE, 68588-0664, USA |
| Email: | nishikant.wase@gmail.com |
| Phone: | 4023109931 |
| Funding Source: | NCESR-704, Nebraska Center for Energy Science Research; EPS-1004094 and 1264409, National Science Foundation ; NSF CBET : 1402896, National Science Foundation |
| Contributors: | Nishikant Wase, Jiri Adamec, Ron Cerny, Girish Rasineni, Paul N Black, Concetta DiRusso |
Summary of all studies in project PR000454
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000621 | Untargeted metabolomic changes in Chlamydomonas reinhardtii treated with lipid inducing small molecules | Chlamydomonas reinhardtii | University of Nebraska-Lincoln | MS* | 2019-01-22 | 1 | 9 | Uploaded data (5.9G)* |
| ST000622 | Identification and metabolite profiling of chemical activators of lipid accumulation in green algae | Chlamydomonas reinhardtii | University of Nebraska-Lincoln | MS* | 2017-10-03 | 1 | 54 | Uploaded data (282.7M)* |
