Summary of project PR000582

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000582. The data can be accessed directly via it's Project DOI: 10.21228/M8FD4K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR000582
Project DOI:doi: 10.21228/M8FD4K
Project Title:Toxoplasma metabolomics
Project Type:Metabolic alterations in wild type vs glycolytic and gluconeogenesis mutants
Project Summary:The focus of this study was to profile the metabolic fates of glucose and glutamine in wild type, glycolytic mutant (hexokinase KO) and gluconeogenesis mutant (PEPCK1 KO) tachyzoite stage T. gondii parasites. 13C6-glucose and 13C5-15N1-glutamine were used as metabolic tracers. Metabolites from glycolysis, pentosephosphate pathway and Kerbs cycle were identified and their isotopomer abundance was quantified in order to visualize metabolic flux across the indicated pathways. A time course (from 0 minutes to 120 minutes) analysis was carried out in the labeling experiments. Here, host cell free extracellular tachyzoite stage T. gondii parasites were used.
Institute:CSIR-National Chemical Laboratory
Department:Biochemical Sciences Division
Laboratory:Molecular Parasitology Laboratory
Last Name:Shanmugam
First Name:Dhanasekaran
Address:Dr. Homi Bhabha Road, Pune, 411008, Maharashtra, India
Email:d.shanmugam@ncl.res.in
Phone:+91-20-25902719

Summary of all studies in project PR000582

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)		 Release
Date		VersionSamplesDownload
(* : Contains raw data)
ST000817 Dynamics of metabolism, proliferation and differentiation in Toxoplasma gondii mutants deficient in glycolysis and gluconeogenesis Toxoplasma gondii CSIR-National Chemical Laboratory MS 2017-10-11 1 54 Uploaded data (594.6M)*
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