Summary of project PR000610
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000610. The data can be accessed directly via it's Project DOI: 10.21228/M8TH6S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000610 |
| Project DOI: | doi: 10.21228/M8TH6S |
| Project Title: | Diet, genetics and gut microbiome drive dynamic changes in plasma metabolites |
| Project Type: | Metabolite profiling of stool and cecal contents in mice of different genotypes, vendors, diets, and antibiotic treatments. |
| Project Summary: | Diet, genetics and the gut microbiome are determinants of metabolic status, in part through production of metabolites by gut microbiota. To understand mechanisms linking these factors, we performed LC-MS-based metabolomic analysis of cecal contents and plasma from C57BL/6J, 129S1/SvImJ and 129S6/SvEvTac mice on chow, high-fat diet (HFD), and HFD-treated with vancomycin or metronidazole. Prediction of the functional metagenome of gut bacteria by PICRUSt analysis of 16S sequences revealed dramatic differences in microbial metabolism. Cecal and plasma metabolites showed multifold differences reflecting the combined and integrated effects of diet, antibiotics, host background, and the gut microbiome. Eighteen plasma metabolites correlated positively or negatively with host insulin resistance across strains and diets. Over 1,000 as-of-yet-unidentified metabolite peaks were also highly regulated by diet, antibiotics and genetic background. Thus diet, host genetics and gut microbiota interact to create unique responses in plasma metabolites, which can contribute to regulation of metabolism and insulin resistance. |
| Institute: | Broad Institute of MIT and Harvard |
| Department: | Metabolomics Platform |
| Last Name: | Avila-Pacheco |
| First Name: | Julian |
| Address: | 415 Main Street, Rm 7175, Cambridge, MA, 02142, USA |
| Email: | jravilap@broadinstitute.org |
| Phone: | 6177148264 |
| Contributors: | Shiho Fujisaka, Julian Avila-Pacheco, Marion Soto, Aleksandar Kostic, Jonathan M. Dreyfuss, Hui Pan, Siegfried Ussar, Emrah Altindis, Ning Li, Lynn Bry, Clary B. Clish, and C. Ronald Kahn |
Summary of all studies in project PR000610
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000879 | Diet, genetics and gut microbiome drive dynamic changes in plasma metabolites [plasma] | Mus musculus | Broad Institute of MIT and Harvard | MS* | 2018-01-29 | 1 | 47 | Uploaded data (18.5G)* |
| ST000880 | Diet, genetics and gut microbiome drive dynamic changes in plasma metabolites [cecal] | Mus musculus | Broad Institute of MIT and Harvard | MS* | 2018-01-29 | 1 | 47 | Uploaded data (19.5G) |
