Summary of project PR000690
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000690. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ3M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000690 |
| Project DOI: | doi: 10.21228/M8GQ3M |
| Project Title: | Single-cell Profiling of Cationic and Anionic Metabolites in Live Frog (Xenopus) Embryos using Microprobe Capillary Electrophoresis Mass Spectrometry |
| Project Type: | Metabolic profiling of anionic and cationic metabolites in single cells |
| Project Summary: | The goal of this study was to enable the analysis of anionic and cationic metabolites from the same identified single cell in Xenopus laevis embryos. A 10 nL portion of identified animal-ventral (V1) cells was aspirated from 8-cell embryos, and metabolites were extracted from the aspirate, before characterization of cationic and anionic compounds using a custom-built capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry platform. A total of ~250 cationic molecular features and ~150 anionic molecular features were detected, including 76 metabolites that were identified in this study. Pathway analysis of the identified metabolites highlighted arginine-proline metabolism of significance. |
| Institute: | University of Maryland |
| Department: | Department of Chemistry & Biochemistry |
| Laboratory: | Nemes Laboratory |
| Last Name: | Nemes |
| First Name: | Peter |
| Address: | 0107 Chemistry Building, 8051 Regents Dr, College Park, MD 20742 |
| Email: | nemes@umd.edu |
| Phone: | 301-405-0373 |
| Funding Source: | National Cancer Institute grant 7R03CA211635 |
Summary of all studies in project PR000690
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001032 | Single-cell Profiling of Cationic and Anionic Metabolites in Live Frog (Xenopus) Embryos using Microprobe Capillary Electrophoresis Mass Spectrometry | Xenopus laevis | University of Maryland | MS | 2019-09-23 | 1 | 6 | Uploaded data (3.9G)* |
