Summary of project PR000691
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000691. The data can be accessed directly via it's Project DOI: 10.21228/M8C10N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000691 |
| Project DOI: | doi: 10.21228/M8C10N |
| Project Title: | A Potent, in vivo Active Antimalarial Series Based on a Triazolopyrazine Core: Open Source Malaria Series 4 |
| Project Summary: | The mode of action of a representative active compound was investigated using an unbiased metabolomics approach, which has previously been shown to reveal both novel and established modes of action of antimalarials (Creek et al 2016, DOI: 10.1128/AAC.01226-16). The active antimalarial OSM-S-313, and the inactive analogue OSM-S-291, were incubated with trophozoite stage P. falciparum parasites for five hours alongside reference compounds including atovaquone (ATV), chloroquine (CQ), dihydroartemisisin (DHA) and three PfATP4 inhibitors, MMV00073, MMV397264 and MMV390482. Metabolomics analysis of cell pellets and spent media allowed reproducible detection of diverse metabolites from a range of metabolic pathways, with the most significant OSM-S-313-induced perturbations observed within peptide, lipid and energy metabolism, suggesting a specific impact on parasite metabolism. |
| Institute: | Monash University |
| Last Name: | Creek |
| First Name: | Darren |
| Address: | Monash Institute of Pharmaceutical Sciences, 381 Royal Parade, Parkville, Melbourne, Victoria, 3052, Australia |
| Email: | darren.creek@monash.edu |
| Phone: | +61 (0) 3 9903 9249 |
Summary of all studies in project PR000691
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001033 | Determination of mode of action of anti-malalrial drugs using untargeted metabolomics | Plasmodium falciparum | Monash University | MS | 2018-08-27 | 1 | 36 | Uploaded data (5.3G)* |
