Summary of project PR000692
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000692. The data can be accessed directly via it's Project DOI: 10.21228/M8769J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000692 |
| Project DOI: | doi: 10.21228/M8769J |
| Project Title: | PAMP-triggered changes in the exometabolome of Arabidopsis suspension cells |
| Project Summary: | The goal of this study was to determine how the exometabolome of defense-elicited Arabidopsis suspension cells inhibits virulence gene expression and growth of a plant pathogenic bacterium Pseudomonas syringae. Arabidopsis T87 suspension cells were treated with the pathogen-associated molecular pattern elf26 or a DMSO-control treatment for six hours, then incubated in fresh media for one hour. The conditioned medium (exudate) was collected from each culture by centrifugation and 0.22 um filter to remove plant cells. These samples are designated T=6 mock and T=6 elf26 in our experimental design. We also prepared samples in the same manner from control-treated cells except without any pre-treatment time prior to one hour exudate production. These samples are labeled T=0 mock. A total of seven biological replicates of each treatment condition were analyzed, with each replicate prepared from cells grown in separate flasks. The exudates were prepared in four independent experiments performed on separate days (1 biological replicate from first experiment, 2 biological replicates from each of the 3 remaining experiments). Four samples of the culture medium, one from each of the four independent experiments, are included. |
| Institute: | Oregon State University |
| Last Name: | Anderson |
| First Name: | Jeff |
| Address: | 2082 Cordley Hall, Corvallis, OR, 97331, USA |
| Email: | anderje2@oregonstate.edu |
| Phone: | 541-737-4076 |
| Funding Source: | NSF 1557694 |
Summary of all studies in project PR000692
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001034 | PAMP-triggered changes in the exometabolome of Arabidopsis suspension cells | Arabidopsis thaliana | Oregon State University | MS* | 2019-03-06 | 1 | 25 | Uploaded data (264.7M)* |
