Summary of project PR000706
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000706. The data can be accessed directly via it's Project DOI: 10.21228/M8DT2N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000706 |
| Project DOI: | doi: 10.21228/M8DT2N |
| Project Title: | Skin and Blubber Metabolomics |
| Project Type: | Multi-institutional Metabolomics |
| Project Summary: | The common bottlenose dolphin (Tursiops truncatus) is carnivorous cetacean thriving in marine environment are one of the most common apex predators found in coastal and estuarine ecosystems. Although recent studies have focused on capturing the circulating metabolomes of these organisms, with respect to pollutants and exposures of the marine environment, the skin and blubber are important protective organs that have not been probed. Using 1HNMR based untargeted metabolomics we quantified 51 metabolites belonging to 74 different metabolic pathways in the skin and blubber of bottle nose dolphins (n=5) samples collected in 2017 from the coast of Mexico. Results indicate that the skin and blubber metabolism are quantitatively different. These metabolite abundances could help discriminate the tissue-types using supervised and unsupervised PCA and PLSDA analysis. Heat maps and random forest analysis point to unique metabolites that are important classifiers of the tissue-type. The altered metabolic patterns, mainly linking fatty acid metabolism and ketogenic amino acids, seem to constitute a characteristic of blubber, while the skin showed diverse metabolites involved in gluceoneogenic pathways. 1H NMR spectra allowed the identification of metabolites associated with these organ types, such as pyruvic acid, arginine, ornithine, 2-hydroxybutyric acid, 3-hydroxyisobutyric acid, and acetic acid, as discriminatory and classifying metabolites. These results would lead to further understanding of dolphin skin and blubber metabolism for better efforts in their conservation as well as a measure of marine pollution and ecotoxicology. |
| Institute: | Wake Forest Baptist Medical Center |
| Department: | Department of Internal Medicine |
| Last Name: | Misra |
| First Name: | Biswapriya |
| Address: | Medical Center Boulevard NRC Building, G#43, Medical Center Boulevard, Winston Salem, NC, 27157, USA |
| Email: | bbmisraccb@gmail.com |
| Phone: | 3522156040 |
| Funding Source: | NA |
| Project Comments: | NA |
| Publications: | In process |
| Contributors: | Biswapriya B. Misra, Ixchel Mariel Ruiz Hernandez, Gloria Ivonne Hernández Bolio, Emanuel Hernandez, Colli Dula Reyna Cristina |
Summary of all studies in project PR000706
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001053 | 1H-NMR Analysis of Skin and Blubber of Nose Dolphin Metabolome Reveal the Functional Metabolomic Dichotomy of The Organs | Tursiops truncatus | Wake Forest Baptist Medical Center | NMR | 2019-12-17 | 1 | 24 | Not available |
