Summary of project PR000710
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000710. The data can be accessed directly via it's Project DOI: 10.21228/M8WT20 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000710 |
| Project DOI: | doi: 10.21228/M8WT20 |
| Project Title: | Improved Extraction method of Polar and Charged Metabolites from Cyanobacteria. |
| Project Summary: | We report excellent extraction method for cyanobacteria of the genus Synechococcus, viz., Synechococcus sp. PCC 7002, Synechococcus elongatus PCC 7942, and a newly isolated Synechococcus elongatus PCC 11801 (manuscript under review). High resolution-LC/MS was used to assess the relative abundance of the extracted metabolites. The different solvent systems used for extraction led to statistically significant changes in the extraction efficiency for a large number of metabolites. While a few hundred m/z features or potential metabolites were detected with different solvent systems, the abundance of over a quarter of all metabolites varied significantly from one solvent system to another. Further, the extraction methods were evaluated for a targeted set of metabolites that are important in 13C-MFA studies of photosynthetic organisms. While for the strain PCC 7002, the reported method using methanol-chloroform-water system gave satisfactory results, a mild base in the form of NH4OH had to be used in place of water to achieve adequate levels of extraction for PCC 7942 and PCC 11801. |
| Institute: | Indian Institute of Technology Bombay |
| Department: | Department of Chemical Engineering |
| Laboratory: | Wangikar Laboratory |
| Last Name: | Wangikar |
| First Name: | Pramod |
| Address: | Room No. 136, Biosystems Lab |
| Email: | wangikar@iitb.ac.in |
| Phone: | 02225764215 |
Summary of all studies in project PR000710
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001057 | Metabolite Extractions from Cyanobacteria | Synechococcus | Indian Institute of Technology Bombay | MS | 2018-09-27 | 1 | 40 | Uploaded data (854.2M)* |
