Summary of project PR000738
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000738. The data can be accessed directly via it's Project DOI: 10.21228/M88X0P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000738 |
| Project DOI: | doi: 10.21228/M88X0P |
| Project Title: | Continuous in vivo metabolism by NMR |
| Project Summary: | Metabolomics relies on analytical methods to provide holistic information about metabolites, their distributions across samples, and their underlying dynamic properties. The latter is gaining increasing attention due to advances in modeling and new analytical methods that provide dense time-series data. We extended high-resolution-magic angle spinning (HR-MAS) NMR—an established technique to measure metabolites from tissues and live organisms—into a flexible, untargeted, and continuous recording of in vivo metabolism. We call this technique “continuous in vivo metabolism by NMR” (CIVM-NMR). We used isotope-edited CIVM-NMR to reproduce a recent amino acid flux result in chronic lymphoid leukemia cells. We then collected untargeted CIVM-NMR datasets for Neurospora crassa, a classic multicellular model of biochemistry, genetics, and metabolism. CIVM-NMR requires virtually no sample preparation and allows for continuous collection of data over hours to days at ~4-min temporal resolution with little noise. CIVM-NMR provided real-time measurements that unambiguously reproduced the direction of flux of branched-chain amino acid accumulation in leukemia cells. It also revealed the dynamics of central carbon metabolism, amino acid metabolism, energy storage molecules, and lipid and cell wall precursors in N. crassa. CIVM-NMR is simple and readily adapted to different types of cells and microorganisms, making it ideally suited to experimentally complement kinetic models of metabolism for diverse biological systems. |
| Institute: | University of Georgia |
| Department: | Genetics; Biochemistry and Molecular Biology |
| Laboratory: | Arthur S. Edison |
| Last Name: | Judge |
| First Name: | Michael |
| Address: | 315 Riverbend Rd., Edison Lab, Athens, GA, 30605, USA |
| Email: | judgemt@uga.edu |
| Phone: | 7046771037 |
| Funding Source: | NSF 1713746; NSF ERC 1648035 (CMaT); Georgia Research Alliance |
| Contributors: | Michael T. Judge, Yue Wu, Fariba Tayyari, John Glushka, Ayuna Hattori, Takahiro Ito, Jonathan Arnold, Arthur S. Edison |
Summary of all studies in project PR000738
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001103 | Continuous in vivo metabolism by NMR | Neurospora crassa | University of Georgia | NMR* | 2019-07-17 | 1 | 6 | Uploaded data (7.7G)* |
