Summary of project PR000740
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000740. The data can be accessed directly via it's Project DOI: 10.21228/M81D57 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000740 |
| Project DOI: | doi: 10.21228/M81D57 |
| Project Title: | Chronic Maternal Cortisol Excess During Late Gestation Leads to Metabolic Alterations in the Newborn Heart |
| Project Summary: | Our laboratory has previously shown in an ovine model of pregnancy that abnormal elevations in maternal cortisol during late gestation lead to increased fetal cardiac arrhythmias and mortality during peripartum. Furthermore, transcriptomic analysis of the fetal heart suggested alterations in TCA cycle intermediates and lipid metabolites in animals exposed to excess cortisol in utero. Therefore, we utilized a sheep model of pregnancy to determine how chronic increases in maternal cortisol alter maternal and fetal serum prior to birth and neonatal cardiac metabolites and lipids at term. Ewes were either infused with 1 mg/kg/day of cortisol starting at gestational day 115 (n=9), or untreated (n=6). Serum was collected from the mother and fetus (125 d-birth), and hearts were collected following birth. Proton nuclear magnetic resonance (1H-NMR) spectroscopy was conducted to measure metabolic profiles of newborn heart specimens as well as fetal and maternal serum specimens. Mass spectrometry was conducted to measure lipid profiles of newborn heart specimens. We observed alterations in amino acid and TCA cycle metabolism as well as lipid and glycerophospholipid metabolism in newborn hearts after excess maternal cortisol in late gestation. In addition, we observed alterations in amino acid and TCA cycle metabolites in fetal but not in maternal serum during late gestation. These results suggest that fetal exposure to excess maternal cortisol alters placental and fetal metabolism prior to birth and limits normal cardiac metabolic maturation, which may contribute to increased risk of peripartum cardiac arrhythmias observed in these animals, or later life cardiomyopathies. |
| Institute: | University of Florida |
| Department: | Pharmacodynamics |
| Last Name: | Keller-Wood |
| First Name: | Maureen |
| Address: | 1345 SW Archer Rd, PO 100487, Gainesville, FL, 32610 |
| Email: | kellerwd@cop.ufl.edu |
| Phone: | NA |
Summary of all studies in project PR000740
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001106 | Lipidomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation (part-1) | Ovis aries | University of Florida | MS | 2019-03-06 | 1 | 12 | Uploaded data (6.2G)* |
| ST001107 | NMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation (part -II) | Ovis aries | University of Florida | NMR | 2019-03-06 | 1 | 36 | Uploaded data (9.9M)* |
| ST001108 | NMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation - Fetal (part -III) | Ovis aries | University of Florida | NMR | 2019-03-06 | 1 | 178 | Uploaded data (83.8M)* |
| ST001109 | NMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation - Maternal (part -IV) | Ovis aries | University of Florida | NMR | 2019-03-06 | 1 | 178 | Uploaded data (83.8M)* |
