Summary of project PR000754
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000754. The data can be accessed directly via it's Project DOI: 10.21228/M8709G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000754 |
| Project DOI: | doi: 10.21228/M8709G |
| Project Title: | Bacteroides-derived sphingolipids are critical for maintaining intestinal homeostasis and symbiosis |
| Project Summary: | Sphingolipids are structural membrane components and important eukaryotic signaling molecules. We hypothesized that sphingolipids mediate intestinal health as they were identified as the most upregulated metabolite feature in stool of inflammatory bowel disease (IBD) patients. Commensal Bacteroidetes also produce sphingolipids, but the impact of these metabolites on host pathways is largely uncharacterized. To study Bacteroidetes sphingolipids in intestinal health, we colonized germ-free mice with a sphingolipid-deficient Bacteroides thetaiotaomicron strain. A lack of Bacteroides-derived sphingolipids increased intestinal inflammation, dysregulated innate immunity and altered the host ceramide pool. Using metabolomic analysis, we described the Bacteroides sphingolipid biosynthesis pathway and revealed a greater variety of Bacteroides-derived sphingolipids than previously recognized, including ceramide phosphoinositol and deoxy-sphingolipids. We annotated Bacteroides sphingolipids in an IBD metabolomic dataset, discovering lower abundances in IBD and negative correlations with gut inflammation and host sphingolipid production. These data highlight the role of sphingolipids in maintaining host-bacterial symbiosis and intestinal homeostasis. |
| Institute: | Broad Institute of MIT and Harvard |
| Last Name: | Avila-Pacheco |
| First Name: | Julian |
| Address: | 415 Main Street, Cambridge MA |
| Email: | jravilap@broadinstitute.org |
| Phone: | 617-714-8264 |
| Funding Source: | National Institutes of Health (P30 DK043351 and R01 AT009708) |
| Contributors: | Eric M. Brown, Xiaobo Ke, Daniel Hitchcock, Timothy D. Arthur, Toru Nakata, Nadine Fornelos, Cortney Heim, Eric A. Franzosa1,4, Curtis Huttenhower1,4, Henry J. Haiser3, Glen 6 Dillow3, Daniel B. Graham1, B. Brett Finlay, Aleksandar D. Kostic, Jeffrey A. Porter, Hera Vlamakis, Sarah Jeanfavre, Julian Avila-Pacheco, Clary B. Clish, and Ramnik J. Xavier |
Summary of all studies in project PR000754
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001125 | WT and ΔSPT cultures of B. thetaiotaomicron and B. ovatus grown BHI liquid media (part I) | Bacteroides thetaiotaomicron VPI-5482;Bacteroides ovatus ATCC 8483 | Broad Institute of MIT and Harvard | MS* | 2019-03-06 | 1 | 8 | Uploaded data (1.1G)* |
| ST001126 | WT and ΔSPT cultures of B. thetaiotaomicron grown in Minimal Media (part II) | Bacteroides thetaiotaomicron VPI-5482 | Broad Institute of MIT and Harvard | MS* | 2019-03-06 | 1 | 6 | Uploaded data (919.2M)* |
| ST001127 | Lipid profiling of caecal samples from GF mice colonized with B. thetaiotaomicron WT or the ΔSPT mutants (part III) | Mus musculus | Broad Institute of MIT and Harvard | MS* | 2019-03-06 | 1 | 14 | Uploaded data (2G)* |
| ST001128 | WT and ΔSPT cultures of B. thetaiotaomicron grown in Minimal Media with or without d4-alanine (part IV) | Bacteroides thetaiotaomicron VPI-5482 | Broad Institute of MIT and Harvard | MS | 2019-03-06 | 1 | 9 | Uploaded data (469M)* |
