Summary of project PR000759
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000759. The data can be accessed directly via it's Project DOI: 10.21228/M8K67W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000759 |
| Project DOI: | doi: 10.21228/M8K67W |
| Project Title: | GC-MS dataset: Leaf metabolic signatures induced by real and simulated herbivory in black mustard (Brassica nigra) |
| Project Type: | Plant metabolomics |
| Project Summary: | Introduction: Methyl jasmonate (MeJA) is an oxylipin hormone active in plant response signalling and defence against herbivory. Although MeJA is applied experimentally to mimic herbivory and induce plant defences, its downstream effects on the plant metabolome are yet largely uncharacterized, especially in the context of plant primary growth and the tissue-specificity of the response. Objectives: We investigated the growth and defence metabolome responses and resistance properties induced by MeJA and real caterpillar herbivory across different leaf stages of the wild annual plant Brassica nigra. Methods: MeJA- and mock-sprayed plants were consecutively treated with or without caterpillars. Foliar cell-bound compounds and herbivore-induced plant volatiles (HIPVs) were analysed combining gas chromatography (GC) and liquid chromatography (LC) time-of-flight mass-spectrometry (TOF/MS). Additionally, gene transcript analyses were performed in Arabidopsis. Results: Plant responses were stronger in young leaves which simultaneously induced higher chlorophyll levels. Both MeJA and caterpillars induced similar, but not identical, accumulation of tricarboxylic acids (TCAs), glucosinolates (GSLs) and phenylpropanoids (PPs), but only caterpillars led to depletion in amino acids. Caterpillars gained less weight when feeding on MeJA-treated plants, indicative of reduced host-plant quality and enhanced resistance. MeJA also caused a three-fold increase in the major defence compound allyl-GSL (sinigrin) upon caterpillar feeding, but did not alter HIPV profiles. Conclusions: The metabolomics approach showed how plant responses induced by real and simulated herbivory extend beyond the regulation of defence metabolism and are tightly modulated throughout leaf development. This leads to a new understanding of the plant metabolic potential that can be further exploited for future plant protection strategies. |
| Institute: | Umeå Plant Science Centre |
| Last Name: | Papazian |
| First Name: | Stefano |
| Address: | Umeå University |
| Email: | stefano.papazian@umu.se |
| Phone: | 0046723160854 |
Summary of all studies in project PR000759
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001134 | GC-TOF-MS, Brassica nigra / MeJA + herbivory | Brassica nigra | Umeå Plant Science Centre | MS | 2019-08-06 | 1 | 55 | Uploaded data (1.7G)* |
