Summary of project PR000818
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000818. The data can be accessed directly via it's Project DOI: 10.21228/M8Z68P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000818 |
| Project DOI: | doi: 10.21228/M8Z68P |
| Project Title: | Vitamin D regulates the microbiota to induce RORgt/FoxP3+ regulatory T cells |
| Project Summary: | The active form of vitamin D (1,25(OH)2D) suppresses experimental models of inflammatory bowel disease in part by regulating the microbiota. In this study, the role of vitamin D in the regulation of microbe induced RORgt/FoxP3+ T regulatory (reg) cells in the colon was determined. Vitamin D sufficient (D+) mice had significantly higher frequencies of FoxP3+ and RORgt/FoxP3+ T reg cells in the colon compared to vitamin D deficient (D-) mice. The higher frequency of RORgt/FoxP3+ T reg cells in D+ colon correlated with higher numbers of bacteria from the Clostridium XIVa and Bacteroides in D+ compared to D- cecum. D- mice with fewer RORgt/FoxP3+ T reg cells were significantly more susceptible to colitis than D+ mice. Transfer of the cecal bacteria from D+ or D- mice to germfree recipients phenocopied the higher numbers of RORgt/FoxP3+ cells and reduced susceptibility to colitis in D+ versus D- recipient mice. 1,25(OH)2D treatment of the D- mice beginning at 3 weeks of age did not completely recover RORgt/FoxP3+ T reg cells or the Bacteriodes, Bacteriodes thetaiotaomicron, and Clostridium XIVa numbers to D+ values. Early vitamin D status shapes the microbiota to optimize the population of colonic RORgt/FoxP3+ T reg cells important for resistance to colitis. |
| Institute: | Pennsylvania State University |
| Last Name: | Nichols |
| First Name: | Robert |
| Address: | 650 toftrees Ave apt 108, state college, PA, 16803 |
| Email: | rgn5011@psu.edu |
| Phone: | 7247662694 |
Summary of all studies in project PR000818
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001219 | Vitamin D regulates the microbiota to induce RORgt/FoxP3+ regulatory T cells | Mus musculus | Pennsylvania State University | NMR | 2019-09-23 | 1 | 63 | Uploaded data (22.2M)* |
