Summary of project PR000841
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000841. The data can be accessed directly via it's Project DOI: 10.21228/M8011M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000841 |
| Project DOI: | doi: 10.21228/M8011M |
| Project Title: | Immunomodulatory activity of hyaluronidase is associated with metabolic adaptations during acute inflammation |
| Project Type: | Untargeted MS |
| Project Summary: | Objective and design: Investigate survival outcomes, and immunological and metabolomic effects of hyaluronidase (Hz) treatment during mouse models of acute inflammation and sepsis. Methods: Survival of C57Bl/6 mice was monitored after lethal challenge with lipopolysaccharide (LPS) or cecal and ligation puncture (CLP)-induced sepsis and treated with Hz or saline. Mice were also challenged with LPS and treated with Hz for leukocyte counting, cytokine quantification and determination of metabolomic profiles in the peritoneal fluid. Results: Hz treatment improved survival outcomes after lethal challenge with LPS or CLPinduced sepsis. LPS challenge promoted acute neutrophil accumulation and production of interleukin-1β (IL-1β) and IL-6 in the peritoneum, whereas Hz treatment suppressed neutrophil infiltration and cytokine production. We further characterized the metabolomic alterations caused by LPS challenge, which predicted activity of metabolic pathways related to fatty acids and eicosanoids. Hz treatment had a profound effect over the metabolic response, reflected by reductions of the relative levels of fatty acids. Conclusion: Collectively, these data demonstrate that Hz treatment is associated with metabolic reprogramming of pathways that sustain the inflammatory response. |
| Institute: | Sao Paulo University |
| Department: | Department of Clinical Analyses, Toxicology and Food Sciences |
| Last Name: | Gardinassi |
| First Name: | Luiz Gustavo |
| Address: | Av do Café s/n - Ribeirão Preto - SP |
| Email: | gustavogardinassi@usp.br |
| Phone: | 551633154189 |
| Funding Source: | FAPESP; CNPq; CAPES |
Summary of all studies in project PR000841
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001255 | Immunomodulatory activity of hyaluronidase is associated with metabolic adaptations during acute inflammation | Mus musculus | Sao Paulo University | MS* | 2019-10-11 | 1 | 40 | Uploaded data (198.4M)* |
