Summary of project PR000936
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000936. The data can be accessed directly via it's Project DOI: 10.21228/M8Q114 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000936 |
| Project DOI: | doi: 10.21228/M8Q114 |
| Project Title: | Chemotaxonomic profiling of Canadian Alternaria populations using high resolution mass spectrometry |
| Project Type: | Alternaria metabolomics |
| Project Summary: | Alternaria spp. occur as plant pathogens worldwide under field and storage conditions. They lead to food spoilage and also produce several classes of secondary metabolites that contaminate the food production chain. From a food safety perspective, the major challenge with assessing the risk of Alternaria contamination is the lack of a clear consensus on their species-level taxonomy. Furthermore, there are currently no reliable DNA sequencing methods to allow for differentiation of the toxigenic potential of these fungi. To address these issues, we performed metabolomic profiling using liquid chromatography high resolution mass spectrometry (LC-HRMS) on 131 Canadian strains of Alternaria to determine their chemotaxonomy. The Alternaria strains were analyzed using principal component analysis (PCA) and unbiased k-means clustering to identify metabolites with significant differences (P < 0.001) between groups. Four populations or ‘chemotypes’ were identified within the strains studied and several known secondary metabolites of Alternaria were identified as distinguishing metabolites. |
| Institute: | Agriculture and Agri-Food Canada |
| Department: | London Research and Development Centre |
| Laboratory: | Natural Products Chemistry |
| Last Name: | Kelman |
| First Name: | Megan |
| Address: | 1391 Sandford Street, London, Ontario N5V 4T3, Canada |
| Email: | megan.kelman@canada.ca |
| Phone: | 519-953-6722 |
| Funding Source: | Agriculture and Agri-Food Canada |
| Contributors: | Megan J. Kelman, Justin B. Renaud, Keith A. Seifert, Jonathan Mack, Ken K.-C. Yeung and Mark W. Sumarah |
Summary of all studies in project PR000936
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001370 | Metabolomic profiling of Canadian species of Alternaria | Alternaria sp. | Agriculture and Agri-Food Canada | MS* | 2020-06-01 | 1 | 338 | Uploaded data (13.2G)* |
