Summary of project PR000944
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000944. The data can be accessed directly via it's Project DOI: 10.21228/M8P41V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000944 |
| Project DOI: | doi: 10.21228/M8P41V |
| Project Title: | Fast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample specific ion distributions |
| Project Summary: | Mass spectrometry based metabolomics is a widely used approach in biotechnology and biomedical research. However, current methods coupling mass spectrometry with chromatography are time-consuming and not suitable for high-throughput analysis of thousands of samples. An alternative approach is flow-injection mass spectrometry (FI-MS) in which samples are directly injected to the ionization source. Here, we show that the sensitivity of Orbitrap FI-MS metabolomics methods is limited by ion competition effect in the detection system. We describe an approach for overcoming this effect by analyzing the distribution of ion m/z values and computationally determining a series of optimal scan ranges. This enables reproducible detection of ~9,000 and ~10,000 m/z features in metabolomics and lipidomics analysis of serum samples, respectively, with a sample scan time of ~15 seconds and duty time of ~30 seconds; a ~50% increase versus current spectral-stitching FI-MS. This approach facilitates high-throughput metabolomics for a variety of applications, including biomarker discovery and functional genomics screens. |
| Institute: | Technion – Israel Institute of Technology |
| Laboratory: | Prof. Tomer Shlomi Lab |
| Last Name: | Shlomi |
| First Name: | Tomer |
| Address: | Technion |
| Email: | tomersh@cs.technion.ac.il |
| Phone: | +972-77-8871497 |
Summary of all studies in project PR000944
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001380 | Fast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample specific ion distributions | Homo sapiens | Technion – Israel Institute of Technology | MS* | 2020-05-22 | 1 | 12 | Uploaded data (6.2G)* |
