Summary of project PR000984
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000984. The data can be accessed directly via it's Project DOI: 10.21228/M8H68N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000984 |
| Project DOI: | doi: 10.21228/M8H68N |
| Project Title: | Evidence for proline utilisation by oral bacterial biofilms grown in saliva |
| Project Type: | Research study |
| Project Summary: | Within the mouth bacteria are starved of saccharides as their main nutrient source between meals and it is unclear what drives their metabolism. Previously oral in vitro biofilms grown in saliva have shown proteolytic degradation of salivary proteins and increased extracellular proline. Although arginine and glucose have been shown before to have an effect on oral biofilm growth and activity, there is limited evidence for proline. Nuclear magnetic resonance (NMR) spectroscopy was used to identify extracellular metabolites produced by bacteria in oral biofilms grown on hydroxyapatite discs. Biofilms were inoculated with whole mouth saliva and then grown for 7 days using sterilised whole mouth saliva supplemented with proline, arginine and glucose as a growth-medium. Overall proline had a beneficial effect on biofilm growth – with significantly fewer dead bacteria present by biomass and surface area of the biofilms (p <0.05). Where arginine and glucose significantly increased and decreased pH, respectively, the pH of proline supplemented biofilms remained neutral at pH 7.3-7.5. SDS-polyacrylamide gel electrophoresis of the spent saliva from proline and arginine supplemented biofilms showed inhibition of salivary protein degradation of immature biofilms. NMR analysis of the spent saliva revealed that proline supplemented biofilms were metabolically similar to unsupplemented biofilms, but these biofilms actively metabolised proline to 5-aminopentanoate, butyrate and propionate, and actively utilised glycine. This study shows that in a nutrient limited environment, proline has a beneficial effect on in vitro oral biofilms grown from a saliva inoculum. |
| Institute: | King's College London |
| Department: | Centre for Host Microbiome Interactions |
| Last Name: | Cleaver |
| First Name: | Leanne |
| Address: | Floor 17, Tower Wing, Guy's Hospital, Great Maze Pond, London, London, SE1 9RT, United Kingdom |
| Email: | leanne.cleaver@kcl.ac.uk |
| Phone: | 07464626438 |
| Funding Source: | This work was funded by a BBSRC Lido-associated PhD studentship for LC in association with Colgate-Palmolive, USA. |
Summary of all studies in project PR000984
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001433 | Evidence for proline utilisation by oral bacterial biofilms grown in saliva | Multi-species non-defined biofilm consortium | King's College London | NMR | 2020-08-03 | 1 | 67 | Uploaded data (49.2M)* |
