Summary of project PR001002
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001002. The data can be accessed directly via it's Project DOI: 10.21228/M85T2G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001002 |
| Project DOI: | doi: 10.21228/M85T2G |
| Project Title: | NMR spectroscopy analysis reveals an altered metabolic homeostasis in Arabidopsis seedlings treated with a cytokinesis inhibitor |
| Project Type: | NMR metabolimics |
| Project Summary: | In plant cytokinesis, de novo formation of a cell plate that evolves into the new cell wall, partitions the cytoplasm of the dividing cell. Cell plate formation involves highly orchestrated vesicle accumulation, fusion, and membrane network maturation supported by the temporary integration of elastic and pliable callose. The small molecule, Endosidin 7 (ES7), arrests late cytokinesis in Arabidopsis by inhibiting callose deposition at the cell plate. Its effect is specific, as it does not broadly affect endomembrane trafficking or cytoskeletal organization. It has emerged as a very valuable tool for dissecting this essential plant process. In order to gain deeper insights regarding its mode of action and the effects of cytokinesis inhibition on overall plant growth, we investigated the effect of ES7 through a nuclear magnetic resonance (NMR) spectroscopy metabolomics approach. In this case study, profiles of Arabidopsis leaf and root tissues were analyzed at different growth stages and ES7 exposure levels. The results show tissue-specific changes in the plant metabolic profile across a developmental gradient, and the effect that ES7 treatment has on the corresponding metabolome. The ES7 induced profile suggests metabolic compensations in central metabolism pathways in response to cytokinesis inhibition. Further, this study shows that long-term treatment of ES7 disrupts the homeostasis of primary metabolism in Arabidopsis seedlings, likely via alteration of hormonal regulation |
| Institute: | California State University Fresno;University of California Davis |
| Department: | Chemistry |
| Last Name: | Krishnan |
| First Name: | Krish |
| Address: | SB-70 Department of Chemistry |
| Email: | krish@csufresno.edu |
| Phone: | 559-278-7944 |
Summary of all studies in project PR001002
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001478 | NMR spectroscopy analysis reveals altered metabolic homeostasis in Arabidopsis seedlings treated with a cytokinesis inhibitor | Arabidopsis thaliana | California State University Fresno | NMR | 2021-06-28 | 1 | 54 | Uploaded data (26.5M)* |
