Summary of project PR001069
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001069. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001069 |
| Project DOI: | doi: 10.21228/M8HQ3X |
| Project Title: | Effect of IPL on E.coli Metabolome hydrophilic phase |
| Project Type: | Untargeted LC-MS metabolomic study |
| Project Summary: | Intense pulsed light (IPL) is becoming a new technical platform for disinfecting food against pathogenic bacteria. Metabolic changes are deemed to occur in bacteria as either the causes or the consequences of IPL-elicited bactericidal and bacteriostatic effects. However, little is known about the influences of IPL on bacterial metabolome. In this study, the IPL treatment was applied to E. coli K-12 for 0-20s, leading to time- and dose-dependent reductions in colony-forming units (CFU) and morphological changes. Cytoplasmic metabolites of the control and IPL-treated E. coli were examined by the liquid chromatography-mass spectrom-etry (LC-MS)-based metabolomic fingerprinting. The results from multivariate modeling and marker identification indicated that the metabolites in redox response, glycolysis, amino acid and nucleotide metabolism were selectively affected by the IPL treatments. |
| Institute: | University of Minnesota |
| Last Name: | Chen |
| First Name: | Chi |
| Address: | 1334 Eckles Ave, St Paul, Minnesota, 55108, USA |
| Email: | chichen@umn.edu |
| Phone: | 6126247704 |
Summary of all studies in project PR001069
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001663 | E.coli K-12 treated by IPL - analysis of polar phase (part-I) | Escherichia coli | University of Minnesota | MS* | 2021-02-17 | 1 | 20 | Uploaded data (255.8M)* |
| ST001664 | E.coli K-12 treated by IPL - analysis of polar phase (part-II) | Escherichia coli | University of Minnesota | MS* | 2021-02-17 | 1 | 20 | Uploaded data (114.9M)* |
