Summary of project PR001073
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001073. The data can be accessed directly via it's Project DOI: 10.21228/M80Q4N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001073 |
| Project DOI: | doi: 10.21228/M80Q4N |
| Project Title: | Reinforcing one-carbon metabolism via folic acid/Folr1 promotes beta-cell formation |
| Project Summary: | Diabetes can be caused by an insufficient beta-cell mass. Here, we performed a genetic screen in a zebrafish model with beta-cell loss to identify pathways promoting beta-cell regeneration. We found that both folate receptor 1 (folr1) overexpression and treatment with folate intermediates, i.e. tetrahydrofolic acid (THF) or folinic acid stimulated beta-cell differentiation in zebrafish. Treatment with these folate intermediates also stimulated beta-cell differentiation in neonatal pig islet cultures, showing the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets, the increased beta-cell differentiation originated from ductal cells. Mechanistically, comparative metabolomics analyses of zebrafish in the basal state vs following -cell ablation and in un-treated vs folinic acid-treated zebrafish indicated beta-cell regeneration could be attributed to changes in the pyrimidine, carnitine and serine pathways. Overall, our results suggest evolutionary conserved and previously unknown roles of folic acid and one-carbon metabolism in the generation of beta-cell . |
| Institute: | North Carolina State University |
| Department: | Molecular and Structural Biochemistry |
| Last Name: | Duan |
| First Name: | Likun |
| Address: | 120 W Broughton Dr. |
| Email: | lduan2@ncsu.edu |
| Phone: | 9195939853 |
Summary of all studies in project PR001073
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001670 | Metabolomics characterization of zebrafish larvae | Danio rerio | North Carolina State University | MS | 2021-03-15 | 1 | 21 | Uploaded data (1.8G)* |
