Summary of project PR001076
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001076. The data can be accessed directly via it's Project DOI: 10.21228/M8MH5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001076 |
| Project DOI: | doi: 10.21228/M8MH5J |
| Project Title: | Targeted Sphingolipid analysis of HeLa silenced for or overexpressing GOLPH3 or LCS |
| Project Summary: | A group of sequentially-acting enzymes operating at the branchpoint among sphingolipid synthetic pathways binds the Golgi-localised oncoprotein GOLPH3. GOLPH3 sorts these enzymes into vesicles for intra-Golgi retro-transport, acting as a component of the cisternae inter-conversion mechanisms. Through these effects, GOLPH3 controls the sub-Golgi localisation, and the lysosomal degradation rate of specific enzymes. Here we evaluated the impact of overexpressing or silencing GOLPH3 or its client enzyme lactosylceramide synthase (LCS) on the sphingolipid composition of HeLa cells by targeted lipid analysis. |
| Institute: | École polytechnique fédérale de Lausanne (EPFL) |
| Department: | IBI |
| Laboratory: | UPDANGELO |
| Last Name: | D'Angelo |
| First Name: | Giovanni |
| Address: | Station 15, Lausanne, Vaud, 1015, Switzerland |
| Email: | giovanni.dangelo@epfl.ch |
| Phone: | +41 216934276 |
Summary of all studies in project PR001076
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001673 | Targeted Sphingolipid analysis of HeLa silenced for or overexpressing GOLPH3 or LCS | Homo sapiens | École polytechnique fédérale de Lausanne (EPFL) | MS | 2021-02-26 | 1 | 15 | Uploaded data (119M)* |
