Summary of project PR001094
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001094. The data can be accessed directly via it's Project DOI: 10.21228/M89394 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001094 |
| Project DOI: | doi: 10.21228/M89394 |
| Project Title: | SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition |
| Project Summary: | Viruses hijack host cell metabolism to acquire the building blocks required for viral replication. Understanding how SARS-CoV-2 alters host cell metabolism could lead to potential treatments for COVID-19, the disease caused by SARS-CoV-2 infection. Here we profile metabolic changes conferred by SARS-CoV-2 infection in kidney epithelial cells and lung air-liquid interface cultures and show that SARS-CoV-2 infection increases glucose carbon entry into the TCA cycle via increased pyruvate carboxylase expression. SARS-CoV-2 also reduces host cell oxidative glutamine metabolism while maintaining reductive carboxylation. Consistent with these changes in host cell metabolism, we show that SARS-CoV-2 increases activity of mTORC1, a master regulator of anabolic metabolism, in cell lines and patient lung stem cell-derived airway epithelial cells. We also show evidence of mTORC1 activation in COVID-19 patient lung tissue. Notably, mTORC1 inhibitors reduce viral replication in kidney epithelial cells and patient-derived lung stem cell cultures. This suggests that targeting mTORC1 could be a useful antiviral strategy for SARS-CoV-2 and treatment strategy for COVID-19 patients, although further studies are required to determine the mechanism of inhibition and potential efficacy in patients. |
| Institute: | University of California, Los Angeles |
| Department: | Biomedical Sciences |
| Laboratory: | Heather Christofk |
| Last Name: | Matulionis |
| First Name: | Nedas |
| Address: | 615 Charles E Young Drive South Los Angeles, CA 90095 |
| Email: | nmatulionis@mednet.ucla.edu |
| Phone: | 310-206-0163 |
Summary of all studies in project PR001094
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001709 | SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition | Homo sapiens | University of California, Los Angeles | MS | 2021-02-24 | 1 | 42 | Uploaded data (3.2G)* |
