Summary of project PR001094

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001094. The data can be accessed directly via it's Project DOI: 10.21228/M89394 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR001094
Project DOI:doi: 10.21228/M89394
Project Title:SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition
Project Summary:Viruses hijack host cell metabolism to acquire the building blocks required for viral replication. Understanding how SARS-CoV-2 alters host cell metabolism could lead to potential treatments for COVID-19, the disease caused by SARS-CoV-2 infection. Here we profile metabolic changes conferred by SARS-CoV-2 infection in kidney epithelial cells and lung air-liquid interface cultures and show that SARS-CoV-2 infection increases glucose carbon entry into the TCA cycle via increased pyruvate carboxylase expression. SARS-CoV-2 also reduces host cell oxidative glutamine metabolism while maintaining reductive carboxylation. Consistent with these changes in host cell metabolism, we show that SARS-CoV-2 increases activity of mTORC1, a master regulator of anabolic metabolism, in cell lines and patient lung stem cell-derived airway epithelial cells. We also show evidence of mTORC1 activation in COVID-19 patient lung tissue. Notably, mTORC1 inhibitors reduce viral replication in kidney epithelial cells and patient-derived lung stem cell cultures. This suggests that targeting mTORC1 could be a useful antiviral strategy for SARS-CoV-2 and treatment strategy for COVID-19 patients, although further studies are required to determine the mechanism of inhibition and potential efficacy in patients.
Institute:University of California, Los Angeles
Department:Biomedical Sciences
Laboratory:Heather Christofk
Last Name:Matulionis
First Name:Nedas
Address:615 Charles E Young Drive South Los Angeles, CA 90095
Email:nmatulionis@mednet.ucla.edu
Phone:310-206-0163

Summary of all studies in project PR001094

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
Release
Date
VersionSamplesDownload
(* : Contains raw data)
ST001709 SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition Homo sapiens University of California, Los Angeles MS 2021-02-24 1 42 Uploaded data (3.2G)*
  logo