Summary of project PR001135
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001135. The data can be accessed directly via it's Project DOI: 10.21228/M80D82 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001135 |
| Project DOI: | doi: 10.21228/M80D82 |
| Project Title: | Multi-omics analysis of glucose-mediated signaling by a moonlighting Gβ protein Asc1/RACK1 |
| Project Type: | C18 Reversed-Phase Broad Spectrum Metabolomics |
| Project Summary: | While much is known about glucose metabolism in yeast, less is known about the receptors and signaling pathways that indicate glucose availability. We obtained metabolic profiles for wildtype and 16 mutants affecting the yeast glucose sensing pathway, comparing 0.05% glucose vs 10 min after glucose addition to 2%. First, we determined that the G protein-coupled receptor (Gpr1/Gpa2) directs early events in glucose utilization while the transceptors (Snf3/Rgt2) regulate subsequent processes and downstream products of glucose metabolism. Whereas the large G protein transmits the signal from its cognate receptor, Ras2 (but not Ras1) integrates responses from both receptor pathways. Second, we determined the relative contributions of the G protein α (Gpa2) and β (Asc1) subunits to glucose-initiated processes. We determined that Gpa2 is primarily involved in regulating carbohydrate metabolism while Asc1 is primarily involved in amino acid metabolism. Both proteins are involved in regulating purine metabolism. Collectively, our analysis reveals the molecular basis for glucose detection and the earliest events of glucose-dependent signal transduction in yeast. |
| Institute: | University of North Carolina at Chapel Hill |
| Department: | Nutrition |
| Laboratory: | Metabolomics and Exposome Laboratory, Nutrition Research Institute, UNC Chapel Hill |
| Last Name: | Sumner |
| First Name: | Susan |
| Address: | 500 Laureate Way, Nutrition Research Institute, UNC Chapel Hill |
| Email: | susan_sumner@unc.edu |
| Phone: | (704) 250-5066 |
Summary of all studies in project PR001135
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001786 | Multi-omics analysis of glucose-mediated signaling by a moonlighting Gβ protein Asc1/RACK1 | Saccharomyces cerevisiae | University of North Carolina at Chapel Hill | MS* | 2021-06-11 | 1 | 191 | Uploaded data (21.8G)* |
