Summary of project PR001140
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001140. The data can be accessed directly via it's Project DOI: 10.21228/M8BQ3C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001140 |
| Project DOI: | doi: 10.21228/M8BQ3C |
| Project Title: | Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature |
| Project Summary: | 1. Decomposition of lignin-rich wood by fungi drives nutrient recycling in woodland ecosystems. Fluctuating abiotic conditions are known to promote the functioning of ecological communities and ecosystems. In the context of wood decay, fluctuating temperature increases decomposition rates. Metabolomics, in tandem with other ‘omics tools, can highlight the metabolic processes affected by experimental treatments, even in the absence of genome sequences and annotations. Globally, natural wood decay communities are dominated by the phylum Basidiomycota. We examined the metabolic responses of Mucidula mucida, a dominant constituent of pioneer communities in beech branches in British woodlands, and Exidia glandulosa, a stress-selected constituent of the same communities, in response to constant and diurnally cycling temperature. 2. We applied untargeted metabolomics and proteomics to beech wood blocks, colonised by M. mucida or E. glandulosa and exposed to either diurnally cycling (mean 15 ± 10°C) or constant (15°C) temperature, in a fully factorial design. 3. Metabolites and proteins linked to lignin breakdown, the citric acid cycle, pentose phosphate pathway, carbohydrate metabolism, fatty acid metabolism and protein biosynthesis and turnover were under-enriched in fluctuating, compared to stable temperatures, in the generalist M. mucida. Conversely E. glandulosa showed little differential response to the experimental treatments. 4. Synthesis. By demonstrating temperature dependant metabolic signatures related to nutrient acquisition in a generalist wood decay fungus, we provide new insights into how abiotic conditions can affect community-mediated decomposition and carbon turnover in forests. We show that mechanisms underpinning important biogeochemical processes can be highlighted using untargeted metabolomics and proteomics in the absence of well-annotated genomes. |
| Institute: | Swansea University |
| Department: | Biosciences |
| Laboratory: | Fungal Molecular Ecology |
| Last Name: | Rawlings |
| First Name: | Anna |
| Address: | Floor 2, Data Science Swansea Medical School, Swansea University, Swansea, Swansea, SA2 8PP, United Kingdom |
| Email: | a.rawlings@swansea.ac.uk |
| Phone: | 07810847321 |
| Funding Source: | NERC grants NE/K011588/1 and NE/K011383/1 |
Summary of all studies in project PR001140
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001805 | Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature | Exidia glandulosa;Mucidula mucida | Swansea University | MS | 2021-06-02 | 1 | 12 | Uploaded data (93.3M)* |
