Summary of project PR001186
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001186. The data can be accessed directly via it's Project DOI: 10.21228/M8DD61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001186 |
| Project DOI: | doi: 10.21228/M8DD61 |
| Project Title: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome |
| Project Type: | Metabolomics to elucidate signaling pathway |
| Project Summary: | Early detection of predators is critical to the survival of all living organisms. For phytoplankton, recognition and response to chemical cues from predators, as evidence of predation risk, is particularly crucial. The phytoplankton Alexandrium minutum upregulates its toxicity when exposed to copepodamides, a suite of compounds released by copepod predators. However, how A. minutum perceives these predatory cues and what metabolic pathways are involved in initiating toxin induction remains unknown. In this study LC/MS and NMR-based metabolomics uncovered subtle physiological responses of A. minutum to copepodamides, including dysregulation of valine biosynthesis and enhancement of butanoate metabolism and arginine biosynthesis. While we have yet to identify a chemoreceptor directly activated by copepod cues, based on the results of inhibition experiments detection of copepodamides appears to disrupt the activity of serine/threonine phosphatases leading to increased jasmonic acid biosynthesis and signaling, which leads to amplified gonyautoxin biosynthesis in A. minutum. This study is an important step toward a better understanding of chemosensory ecology of predator-prey interactions in phytoplankton. |
| Institute: | Georgia Institute of Technology |
| Department: | School of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience |
| Laboratory: | Kubanek Lab |
| Last Name: | Brown |
| First Name: | Emily |
| Address: | 950 Atlantic Dr Atlanta, GA, 30332, USA |
| Email: | julia.kubanek@biosci.gatech.edu |
| Phone: | 404-894-8424 |
| Project Comments: | This study has 3 parts: 2 NMR (polar and non-polar metabolites) and MS |
| Contributors: | Emily R. Brown, Sam G. Moore, David A. Gaul, and Julia Kubanek |
Summary of all studies in project PR001186
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001880 | NMR Predator Cues Target Signaling Pathways in Toxic Algal Metabolome (Polar metabolites) | Alexandrium minutum | Georgia Institute of Technology | NMR* | 2021-07-27 | 1 | 40 | Uploaded data (33.4M)* |
| ST001881 | NMR Predator Cues Target Signaling Pathways in Toxic Algal Metabolome (Non-polar metabolites) | Alexandrium minutum | Georgia Institute of Technology | NMR* | 2021-07-27 | 1 | 40 | Uploaded data (33.4M)* |
| ST001882 | LC-MS for Predator Cues Target Signaling Pathways in Toxic Algal Metabolome Protocol | Alexandrium minutum | Georgia Institute of Technology | MS* | 2021-07-27 | 1 | 49 | Uploaded data (12.6G)* |
