Summary of project PR001188
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001188. The data can be accessed directly via it's Project DOI: 10.21228/M84X4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001188 |
| Project DOI: | doi: 10.21228/M84X4Z |
| Project Title: | Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome |
| Project Summary: | Recognition and rejection of chemically defended prey is critical to maximizing fitness for predators. Paralytic shellfish toxins (PSTs) which strongly inhibit voltage-gated sodium channels in diverse animal taxa are produced by several species of the bloom-forming algal genus Alexandrium where they appear to function as chemical defenses against grazing copepods. Despite PSTs being produced and localized within phytoplankton cells, some copepods distinguish toxic from non-toxic prey, selectively ingesting less toxic cells, in ways that suggest cell surface recognition perhaps associated with non-polar metabolites. In this study LC/MS and NMR-based metabolomics revealed that the non-polar metabolomes of two toxic species (Alexandrium catenella and Alexandrium pacificum) vary considerably from their non-toxic congener Alexandrium tamarense despite all three being very closely related. Toxic and non-toxic Alexandrium spp. were distinguished from each other by metabolites belonging to seven lipid classes. Of these, 17 specific metabolites were significantly more abundant in both toxic A. catenella and A. pacificum compared to non-toxic A. tamarense suggesting that just a small portion of the observed metabolic variability is associated with toxicity. Future experiments aimed at deciphering chemoreception mechanisms of copepod perception of Alexandrium toxicity should consider these metabolites, and the broader lipid classes phosphatidylcholines and sterols, as potential candidate cues. |
| Institute: | Georgia Institute of Technology |
| Last Name: | Brown |
| First Name: | Emily |
| Address: | 950 Atlantic Dr Atlanta GA 30332, USA |
| Email: | julia.kubanek@biosci.gatech.edu |
| Phone: | 404-894-8424 |
Summary of all studies in project PR001188
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001885 | MS Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome | Alexandrium catenella;Alexandrium tamarense;Alexandrium pacificum | Georgia Institute of Technology | MS* | 2021-08-09 | 1 | 70 | Uploaded data (1.8G)* |
| ST001895 | NMR Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome (Experiment 1) | Alexandrium catenella; Alexandrium tamarense | Georgia Institute of Technology | NMR* | 2021-08-09 | 1 | 30 | Uploaded data (31.8M)* |
| ST001896 | NMR Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome (Experiment 2) | Alexandrium pacificum; Alexandrium tamarense | Georgia Institute of Technology | NMR* | 2021-08-09 | 1 | 30 | Uploaded data (31.7M)* |
