Summary of project PR001195
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001195. The data can be accessed directly via it's Project DOI: 10.21228/M87M55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001195 |
| Project DOI: | doi: 10.21228/M87M55 |
| Project Title: | Systemic host inflammation induces stage-specific transcriptomic modification and slower maturation in malaria parasites |
| Project Type: | MS untargeted metabolomics analysis |
| Project Summary: | Previous reports suggest that the maturation rate of malaria parasites within red blood cells (RBC) is not constant for a given species in vivo. For instance, maturation can be influenced by host nutrient status or circadian rhythm. Here we observed in mice that systemic host inflammation, induced by lipopolysaccharide (LPS) conditioning or ongoing acute malaria infection, slowed the progression of a single cohort of parasites from one generation of RBC to the next. LPS-conditioning and acute infection both triggered substantial changes to the metabolomic composition of plasma in which parasites circulated. This altered plasma directly slowed parasite maturation in a manner that could not be rescued by supplementation, consistent with the presence of inhibitory factors. Single-cell transcriptomic assessment of mixed parasite populations, exposed to a short period of systemic host inflammation in vivo, revealed specific impairment in the transcriptional activity and translational capacity of trophozoites compared to rings or schizonts. Thus, we provide in vivo evidence of transcriptomic and phenotypic plasticity of asexual blood-stage Plasmodium parasites when exposed to systemic host inflammation |
| Institute: | QIMR Berghofer Medical Research Institute |
| Department: | Cell & Molecular Biology Department |
| Laboratory: | Precision & Systems Biomedicine |
| Last Name: | Stoll |
| First Name: | Thomas |
| Address: | 300 Herston Road, Herston QLD 4006, Australia |
| Email: | thomas.stoll@qimrberghofer.edu.au |
| Phone: | +61 7 3845 3992 |
Summary of all studies in project PR001195
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001899 | Systemic host inflammation induces stage-specific transcriptomic modification and slower maturation in malaria parasites (part I) | Mus musculus | QIMR Berghofer Medical Research Institute | MS* | 2023-06-26 | 1 | 39 | Uploaded data (9.1G)* |
| ST001900 | Systemic host inflammation induces stage-specific transcriptomic modification and slower maturation in malaria parasites (part II) | Mus musculus | QIMR Berghofer Medical Research Institute | MS* | 2023-06-26 | 1 | 39 | Uploaded data (9.1G)* |
