Summary of project PR001259
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001259. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX2N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001259 |
| Project DOI: | doi: 10.21228/M8ZX2N |
| Project Title: | Metabolomic Fingerprinting of Human High Grade Serous Ovarian Carcinoma Cell Lines |
| Project Type: | Cell line analysis |
| Project Summary: | Focusing on defining the metabolomic basis of intratumoral heterogeneity in ovarian cancer, the metabolic diversity of a panel of high grade serous ovarian carcinoma (HGSOC) cell-lines we investigated using a metabolomics platform that interrogate 731 compounds. Metabolic fingerprinting followed by 2-dimensional and 3-dimensional principal component analysis defined the heterogeneity of the HGSOC cells and clustered them into five distinct metabolic groups. An overall increase in the metabolites associated with aerobic glycolysis and phospholipid metabolism were observed in the majority of the cancer cells. A preponderant increase in the levels of metabolites involved in trans-sulfuration and glutathione synthesis was also observed. Subsets of HGSOC cells showed an increase in the levels of 5-Hydroxytryptamine, gamma-aminobutyric acid, or glutamate, pointing to their potential role as oncometabolites. In summary, our results identify increased glycolysis, phospholipid metabolism and amino acid metabolism with the resultant increase in the levels of 5-Hydoxytryptamine, GABA, and Glutamate as metabolomic correlates underlying the heterogeneity of ovarian cancer cell lines. |
| Institute: | University of Oklahoma Health Sciences Center |
| Department: | Cell Biology |
| Laboratory: | Danny N Dhanasekaran |
| Last Name: | Jayaraman |
| First Name: | Muralidharan |
| Address: | 975 NE 10th street, BRC1470, Oklahoma City, Oklahoma, 73104, USA |
| Email: | Muralidharan-Jayaraman@ouhsc.edu |
| Phone: | 4052718001 x 30492 |
| Contributors: | Danny N. Dhanasekaran, Jihee Ha, Padmaja Dhanasekaran, Mingda Yan |
Summary of all studies in project PR001259
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001983 | Metabolomic Fingerprinting of Human High Grade Serous Ovarian Carcinoma Cell Lines | Homo sapiens | University of Oklahoma Health Sciences Center | MS | 2021-12-01 | 1 | 30 | Not available |
