Summary of project PR001264
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001264. The data can be accessed directly via it's Project DOI: 10.21228/M8B39F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001264 |
| Project DOI: | doi: 10.21228/M8B39F |
| Project Title: | Metabolomics of the interaction between a consortium of entomopathogenic fungi and their target insect: mechanisms of attack and survival |
| Project Type: | Untargeted Metabolomics |
| Project Summary: | One of the most concerning pests that attack strawberries in Brazil is Duponchelia fovealis, a non-native moth with no registered control methods to date. Our group recently observed that a fungal consortium formed by two strains of Beauveria bassiana increased the mortality of D. fovealis more than inoculation with each strain on its own. However, the molecular interaction between the fungal consortium and the caterpillars is unknown, raising several questions about the enhanced pest control observed. Furthermore, concerns over the emergency of resistance and the selection for resistance to chemical and biological products that are constantly applied in agriculture highlight the need for careful examination of novel pest control methods. Thus, in this work, we sought to pioneer the evaluation of the molecular interaction between a fungal consortium of B. bassiana and D. fovealis caterpillars. We aimed to understand the biocontrol process involved in this interaction and the defense system of the caterpillar. Therefore, seven days after D. fovealis caterpillars were inoculated with the B. bassiana consortium, the dead and surviving caterpillars were analyzed using GC-MS and LC-MS/MS. |
| Institute: | Universidade Federal do Paraná |
| Department: | Patologia Básica |
| Laboratory: | Laboratório de Microbiologia e Biologia Molecular |
| Last Name: | Katiski da Costa Stuart |
| First Name: | Andressa |
| Address: | Av. Cel. Francisco Heráclito dos Santos, 100, Curitiba, Paraná, 81530-000, Brazil |
| Email: | andressa.katiski@gmail.com |
| Phone: | 5541991922779 |
| Project Comments: | Previous studies by our research group showed the increased potential for biocontrol on the insect pest Duponchelia fovealis when two strains of Beauveria bassiana were used together as a fungal consortium. In this work we sought to identify the metabolites involved in the interaction between the consortium formed by different B. bassiana strains and its target insect, D. fovealis. We identify the metabolites using non-targeted metabolomics, applying gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS). These analyses aimed to elucidate the molecular mechanisms involved in the biocontrol effect of the fungal consortium on D. fovealis and to examine possible explanations for the survival of some caterpillars due to a potential resistance mechanism. Dead and surviving caterpillars were analyzed separately. |
| Contributors: | Jason Lee Furuie, Thais Regiani Cataldi, Rodrigo Makowiecky Stuart, Maria Aparecida Cassilha Zawadneak, Carlos Alberto Labate, Ida Chapaval Pimentel |
Summary of all studies in project PR001264
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001990 | Metabolomics of the interaction between a consortium of entomopathogenic fungi and their target insect: mechanisms of attack and survival | Duponchelia fovealis | Universidade Federal do Paraná | MS* | 2023-05-12 | 1 | 35 | Uploaded data (13.3G)* |
