Summary of project PR001268
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001268. The data can be accessed directly via it's Project DOI: 10.21228/M8T39S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001268 |
| Project DOI: | doi: 10.21228/M8T39S |
| Project Title: | Polyamine import and accumulation causes immunomodulation in macrophages engulfing apoptotic cells |
| Project Summary: | Phagocytosis of apoptotic cells, termed efferocytosis, is critical for tissue homeostasis and drives anti-inflammatory programming in engulfing macrophages. Here, we assess metabolites in naïve and inflammatory macrophages following engulfment of multiple cellular and non-cellular targets. Efferocytosis leads to unique increases in the arginine-derived polyamines, spermidine and spermine, in vitro and in vivo. Surprisingly, polyamine accumulation after efferocytosis does not arise from retention of apoptotic cell metabolites or de novo synthesis, but from enhanced polyamine import that is dependent on Rac1, actin, and PI3 kinase. Blocking polyamine import prevents efferocytosis from suppressing macrophage IL-1or IL-6. This identifies efferocytosis as a trigger for polyamine import and accumulation, and imported polyamines as mediators of efferocytosis-induced immune reprogramming. |
| Institute: | University of Colorado Denver |
| Last Name: | Haines |
| First Name: | Julie |
| Address: | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| Email: | julie.haines@cuanschutz.edu |
| Phone: | 3037243339 |
Summary of all studies in project PR001268
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001996 | Polyamine import and accumulation causes immunomodulation in macrophages engulfing apoptotic cells (Part 1) | Mus musculus | University of Colorado Denver | MS | 2021-12-08 | 1 | 18 | Uploaded data (802.6M)* |
| ST001997 | Polyamine import and accumulation causes immunomodulation in macrophages engulfing apoptotic cells (Part 2) | Mus musculus | University of Colorado Denver | MS | 2021-12-08 | 1 | 18 | Uploaded data (1.5G)* |
| ST001998 | Polyamine import and accumulation causes immunomodulation in macrophages engulfing apoptotic cells (Part 3) | Mus musculus | University of Colorado Denver | MS | 2021-12-08 | 1 | 9 | Uploaded data (346.6M)* |
| ST001999 | Polyamine import and accumulation causes immunomodulation in macrophages engulfing apoptotic cells (Part 4) | Mus musculus | University of Colorado Denver | MS | 2021-12-08 | 1 | 9 | Uploaded data (820M)* |
