Summary of project PR001278
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001278. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001278 |
| Project DOI: | doi: 10.21228/M8HQ49 |
| Project Title: | PolyFerm |
| Project Summary: | The increased prevalence of Salmonella spp. resistance in swine spurs the search for alternatives to antibiotics. Microcin J25 (MccJ25), a bacteriocin produced by Escherichia coli, is a potent inhibitor of several pathogenic bacteria including Salmonella enterica. In this study, we aimed to evaluate in vitro the impact of MccJ25 on the metabolic activity of the swine colonic microbiota. The PolyFermS in vitro continuous fermentation model was used with modified Macfarlane medium to simulate the porcine proximal colon. During 35 days of fermentation, a first-stage reactor containing immobilized swine fecal microbiota fed two second-stage control and test reactors operated in parallel and used to test the effectsof MccJ25 on the composition and the metabolic activity of the microbiota. Reuterin, a broad spectrum antimicrobial produced by Limosilactobacillus reuteri and the antibiotic rifampicin were tested for comparison. LC-MS analysis of the cell extracts was used to assess the bacteriocin/antibiotic degradation products and monitor changes in the swine colonic microbiota metabolome. |
| Institute: | National Museum of Natural History |
| Laboratory: | Communication Molecules and Adaptation of Microorganisms |
| Last Name: | Zirah |
| First Name: | Severine |
| Address: | Museum national d'Histoire naturelle, Unité MCAM UMR 7245 CNRS-MNHN, CP 54, 57 rue Cuvier, 75005 Paris, FRANCE |
| Email: | severine.zirah@mnhn.fr |
| Phone: | +33(0)1 40 79 31 40 |
Summary of all studies in project PR001278
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002014 | Impact of microcin J25 on the porcine microbiome in a continuous culture model | swine colonic microbiota | National Museum of Natural History | MS* | 2022-09-14 | 1 | 197 | Uploaded data (222.3G)* |
