Summary of project PR001302
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001302. The data can be accessed directly via it's Project DOI: 10.21228/M8DQ4C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001302 |
| Project DOI: | doi: 10.21228/M8DQ4C |
| Project Title: | Distinct Human Hepatocyte Lipidomes for Nonalcoholic Steatohepatitis and In Vitro-Induced Steatosis |
| Project Summary: | Nonalcoholic steatohepatitis (NASH) is a severe form of steatotic liver injury that can be caused by a variety of stimuli and has a significant mortality rate. A common technique to induce in vitro steatosis involves culturing primary human hepatocytes (PHH) in a fatty acid-enriched media. This study compared the lipidome of PHH cultured in a fatty acid-enriched media to hepatocytes from patients with NASH and healthy controls to determine whether such culture techniques could generate a hepatocellular lipid profile similar to that observed in NASH patients. LC-MS lipidomics analysis of hepatocytes from patients with NASH revealed increases in the total cellular abundance of glycerolipids, phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols and phosphatidylserines compared to healthy control hepatocytes. PHH cultured in a fatty acid-enriched environment demonstrated an increase in total lipid abundance, however, changes were limited to glycerolipids; in contrast to NASH hepatocytes, increases in the abundance of phospholipids were not observed. |
| Institute: | Monash Institute of Pharmaceutical Sciences |
| Last Name: | Kralj |
| First Name: | Thomas |
| Address: | 381 Royal Parade |
| Email: | Tom.Kralj@monash.edu |
| Phone: | +61 3 99026000 |
Summary of all studies in project PR001302
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002057 | Distinct Human Hepatocyte Lipidomics Profiles for Nonalcoholic Steatohepatitis and In Vitro-Induced Steatosis | Homo sapiens | Monash Institute of Pharmaceutical Sciences | MS | 2022-01-25 | 1 | 103 | Uploaded data (18.5G)* |
