Summary of project PR001304
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001304. The data can be accessed directly via it's Project DOI: 10.21228/M8570V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001304 |
| Project DOI: | doi: 10.21228/M8570V |
| Project Title: | Pollen metabolomics using Arabidopsis thaliana: Comparison of pollen at mature, hydration and germination stage |
| Project Type: | Untargeted Metabolomics |
| Project Summary: | Pollen germination is an essential process for pollen tube growth, pollination, and therefore seed production in flowing plants, which requires energy either from remobilization of stored carbon sources, lipid and starch, or from secreted exudates from stigma. Transcriptome analysis from in vitro pollen germination previously showed 14 GO terms, including metabolism and energy, which are overrepresented in Arabidopsis. However, the global changes of carbohydrate and energy-related metabolites during the transition from mature pollen grain to hydrated pollen, the prerequisite step to pollen germination, is poorly understood in Arabidopsis. In this study, we investigated the differential metabolic pathway enrichment among mature, hydrated, and germinated pollen using untargeted metabolomics analysis. Integration of publicly available transcriptome with presented metabolome data revealed starch and sucrose metabolism was significantly increased during pollen hydration and germination. The alterations in central metabolism focusing on sugar, fatty acids, and lipids were analyzed in detail. Several metabolites, including palmitic acid, oleic acid, linolenic acid, quercetin, luteolin/kaempferol, and γ-aminobutyric acid (GABA), were elevated in the hydrated pollen, suggesting a potential role in activating pollen tube emergence. The metabolite levels of mature, hydrated, and germinated pollen, presented in this work provide insights on the molecular basis of pollen germination. |
| Institute: | Donald Danforth Plant Science Center |
| Department: | Allen/USDA lab |
| Laboratory: | Doug K. Allen |
| Last Name: | Kambhampati |
| First Name: | Shrikaar |
| Address: | 975 North Warson Road, St. Louis, MO 63132 |
| Email: | shrikaar.k@gmail.com |
| Phone: | 3144025550 |
| Funding Source: | NIH, USDA-ARS |
| Contributors: | Shrikaar Kambhampati, Jiang Wang, Doug Allen, Li-Qing Chen |
Summary of all studies in project PR001304
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002060 | Pollen metabolomics using Arabidopsis thaliana: Comparison of pollen at mature, hydration and germination stage | Arabidopsis thaliana | University of Illinois Urbana-Champaign | MS* | 2022-02-07 | 1 | 72 | Uploaded data (1.2G)* |
