Summary of project PR001312
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001312. The data can be accessed directly via it's Project DOI: 10.21228/M8499N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001312 |
| Project DOI: | doi: 10.21228/M8499N |
| Project Title: | Lipidomic Comparison of 2D and 3D Colon Cancer Cell Culture Models |
| Project Type: | Untargeted Lipidomics |
| Project Summary: | Altered lipid metabolism is one of the hallmarks of cancer. Cellular proliferation and de novo synthesis of lipids are related to cancer progression. In this study, we evaluated the lipidomic profile of two-dimensional (2D) monolayer and multicellular tumor spheroids from the HCT 116 colon carcinoma cell line. We utilized serial trypsinization on the spheroid samples to generate three cellular populations representing the proliferative, quiescent, and necrotic regions of the spheroid. This analysis enabled a comprehensive identification and quantification of lipids produced in each of the spheroid layer and 2D cultures. We show that lipid subclasses associated with lipid droplets form in oxygen-restricted and acidic regions of spheroids and are produced at higher levels than in 2D cultures. Additionally, sphingolipid production, which is implicated in cell death and survival pathways, is higher in spheroids relative to 2D cells. Finally, we show that increased numbers of lipids comprised of polyunsaturated fatty acids (PUFAs) are produced in the quiescent and necrotic regions of the spheroid. The lipidomic signature for each region and cell culture type highlights the importance of understanding the spatial aspects of cancer biology. These results provide additional lipid biomarkers in the tumor microenvironment that can be further studied during potential therapeutic studies which target pivotal lipid production pathways. |
| Institute: | The Ohio State University |
| Department: | Department of Chemistry and Biochemistry |
| Laboratory: | Amanda B Hummon Research Group |
| Last Name: | Tobias |
| First Name: | Fernando |
| Address: | 100 W 18th Avenue, Columbus, Ohio, 43210, USA |
| Email: | tobias.62@osu.edu |
| Phone: | 1234567890 |
Summary of all studies in project PR001312
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002070 | Lipidomic Comparison of 2D and 3D Colon Cancer Cell Culture Models | Homo sapiens | The Ohio State University | MS | 2022-02-14 | 1 | 59 | Uploaded data (17.1G)* |
