Summary of project PR001312

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001312. The data can be accessed directly via it's Project DOI: 10.21228/M8499N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR001312
Project DOI:doi: 10.21228/M8499N
Project Title:Lipidomic Comparison of 2D and 3D Colon Cancer Cell Culture Models
Project Type:Untargeted Lipidomics
Project Summary:Altered lipid metabolism is one of the hallmarks of cancer. Cellular proliferation and de novo synthesis of lipids are related to cancer progression. In this study, we evaluated the lipidomic profile of two-dimensional (2D) monolayer and multicellular tumor spheroids from the HCT 116 colon carcinoma cell line. We utilized serial trypsinization on the spheroid samples to generate three cellular populations representing the proliferative, quiescent, and necrotic regions of the spheroid. This analysis enabled a comprehensive identification and quantification of lipids produced in each of the spheroid layer and 2D cultures. We show that lipid subclasses associated with lipid droplets form in oxygen-restricted and acidic regions of spheroids and are produced at higher levels than in 2D cultures. Additionally, sphingolipid production, which is implicated in cell death and survival pathways, is higher in spheroids relative to 2D cells. Finally, we show that increased numbers of lipids comprised of polyunsaturated fatty acids (PUFAs) are produced in the quiescent and necrotic regions of the spheroid. The lipidomic signature for each region and cell culture type highlights the importance of understanding the spatial aspects of cancer biology. These results provide additional lipid biomarkers in the tumor microenvironment that can be further studied during potential therapeutic studies which target pivotal lipid production pathways.
Institute:The Ohio State University
Department:Department of Chemistry and Biochemistry
Laboratory:Amanda B Hummon Research Group
Last Name:Tobias
First Name:Fernando
Address:100 W 18th Avenue, Columbus, Ohio, 43210, USA
Email:tobias.62@osu.edu
Phone:1234567890

Summary of all studies in project PR001312

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
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(* : Contains raw data)
ST002070 Lipidomic Comparison of 2D and 3D Colon Cancer Cell Culture Models Homo sapiens The Ohio State University MS 2022-02-14 1 59 Uploaded data (17.1G)*
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