Summary of project PR001329
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001329. The data can be accessed directly via it's Project DOI: 10.21228/M8XM74 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001329 |
| Project DOI: | doi: 10.21228/M8XM74 |
| Project Title: | Lipidomics of High Fat vs Control Mice |
| Project Type: | Untargeted LC-MS/MS Lipidomics |
| Project Summary: | Obesity exacerbates inflammation upon lung injury; however, the mechanisms by which obesity primes pulmonary dysregulation prior to injury are not well studied. Notably, little is known about how obesity dysregulates pulmonary polyunsaturated fatty acid (PUFA) metabolism that is central to inflammation initiation and resolution. Herein, we first show with mass spectrometry that a high fat diet (HFD) administered to C57BL/6J mice increases the relative abundance of pulmonary PUFA-containing triglycerides and the concentration of PUFA-derived oxylipins, independent of an increase in total pulmonary PUFAs. Experiments with a genetic model of obesity did not recapitulate the effects of the HFD on the pulmonary oxylipin signature, suggesting a diet-driven effect. Subsequent pulmonary next-generation RNA sequencing showed complex and unique transcriptional regulation with a HFD. The HFD increased pathways related to glycerophospholipid metabolism, innate immunity, and inflammation including an elevation in B cell differentiation and signaling. Finally, computational integration of lipidomic with transcriptomic data revealed novel networks with the HFD between glycerophospholipid metabolism and B cell receptor signaling with specific oxylipins. Collectively, these data show obesity dysregulates pulmonary PUFA metabolism prior to lung injury, which may be a mechanism by which obesity primes the lungs to respond poorly upon infectious and/or inflammatory challenges. |
| Institute: | University of North Carolina at Chapel Hill |
| Department: | Chemistry |
| Laboratory: | MS Core Laboratory |
| Last Name: | Weatherspoon |
| First Name: | Emily |
| Address: | 131 South Rd |
| Email: | emdiane@email.unc.edu |
| Phone: | 7042453664 |
Summary of all studies in project PR001329
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002093 | Lipidomics of High Fat vs Control Mice | Mus musculus | University of North Carolina at Chapel Hill | MS | 2022-03-15 | 1 | 13 | Not available |
