Summary of project PR001355
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001355. The data can be accessed directly via it's Project DOI: 10.21228/M8K70K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001355 |
| Project DOI: | doi: 10.21228/M8K70K |
| Project Title: | Mitochondrial respiration in B lymphocytes is essential for humoral immunity by controlling flux of the TCA cycle |
| Project Summary: | The function of mitochondrial respiration during B cell fate decisions and differentiation 55 remained equivocal. This study reveals that selection for mitochondrial fitness occurs during B 56 cell activation and is essential for subsequent plasma cell differentiation. By expressing a 57 mutated mitochondrial helicase in transitional B cells, we depleted mitochondrial DNA during 58 B cell maturation, resulting in reduced oxidative phosphorylation. Although no changes in 59 follicular B cell development were evident, germinal centers, class switch recombination to 60 IgG, plasma cell maturation and humoral immunity were diminished. Defective oxidative 61 phosphorylation led to aberrant flux of the tricarboxylic acid cycle and lowered the amount of 62 saturated phosphatidic acid. Consequently, mTOR activity and BLIMP1 induction were 63 curtailed whereas HIF1 _and glycolysis were amplified. Exogenous phosphatidic acid 64 increased mTOR activity in activated B cells. Hence, mitochondrial function is required and 65 selected for in activated B cells for the successful generation of functional plasma cells. |
| Institute: | University of Erlangen-Nuremberg Chair of Biochemistry |
| Last Name: | Hofmann |
| First Name: | Joerg |
| Address: | Staudtstr.5, Erlangen, Bavaria, 91058, Germany |
| Email: | joerg.hofmann@fau.de |
| Phone: | +49 9131 85 8260 |
Summary of all studies in project PR001355
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002140 | Mitochondrial respiration in B lymphocytes is essential for humoral immunity by controlling flux of the TCA cycle | Mus musculus | University of Erlangen-Nürnberg | MS | 2022-05-02 | 1 | 29 | Uploaded data (29.9M)* |
