Summary of project PR001363
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001363. The data can be accessed directly via it's Project DOI: 10.21228/M8J708 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001363 |
| Project DOI: | doi: 10.21228/M8J708 |
| Project Title: | Sphingomyelin depletion inhibits CXCR4 dynamics and CXCL12-mediated directed cell migration in human T cells |
| Project Summary: | Sphingolipids, ceramides and cholesterol are integral components of cellular membranes, and they also play important roles in signal transduction by regulating the dynamics of membrane receptors through their effects on membrane fluidity. Here, we combined biochemical and functional assays with single-molecule dynamic approaches to demonstrate that the local lipid environment regulates CXCR4 organization and function and modulates chemokine-triggered directed cell migration. Prolonged treatment of T cells with neutral sphingomyelinase promoted the complete and sustained breakdown of sphingomyelins and the accumulation of the corresponding ceramides, which altered both membrane fluidity and CXCR4 nanoclustering and dynamics. Under these conditions CXCR4 retained some CXCL12-mediated signaling activity but failed to promote efficient directed cell migration. Our data underscore a critical role for the local lipid composition at the cell membrane in regulating the lateral mobility of chemokine receptors, and their ability to dynamically increase receptor density at the leading edge to promote efficient cell migration. |
| Institute: | Universidad CEU San Pablo |
| Department: | Center of Metabolomics and Bioanalysis |
| Last Name: | Gonzalez-Riano |
| First Name: | Carolina |
| Address: | km 0, Universidad CEU-San Pablo Urbanización Montepríncipe. M-501 |
| Email: | carolina.gonzalezriano@ceu.es |
| Phone: | 646251045 |
Summary of all studies in project PR001363
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002150 | Sphingomyelin depletion inhibits CXCR4 dynamics and CXCL12-mediated directed cell migration in human T cells | Homo sapiens | Universidad CEU San Pablo | MS | 2022-05-09 | 1 | 27 | Uploaded data (10.3G)* |
