Summary of project PR001409
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001409. The data can be accessed directly via it's Project DOI: 10.21228/M8KT4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001409 |
| Project DOI: | doi: 10.21228/M8KT4Z |
| Project Title: | Lipolysis-derived Lipids Determine Autophagy Initiation during Fasting |
| Project Summary: | For survival, autophagy is a crucial intracellular self-degradation process to provide energy sources, helping adapt to nutrient deprivation. Although nutrient availability is a key determinant of autophagy initiation, it remains elusive underlying mechanism(s) of perceiving nutritional scarcity by which cells timely turn on autophagy as the last self-destructive process for energy supply. Here, we showed that PKA-dependent lipolysis can block the initiation of futile autophagy during short-term nutritional deprivation by repressing AMPK. Using Raman microscopy imaging and metabolomics, we found that autophagy occurred by reduction in available free fatty acids (FFAs) for energy sources. By modulating genes involved in lipolysis and fatty acid oxidation, we found that the use of lipolysis-derived FFAs precedes autophagy initiation. The dysregulated autophagy suppression during short-term fasting decreased motility and lifespan extension of worms. Taken together, these data suggest that PKA is a pivotal factor to orchestrate sophisticated catabolic pathways, preferring the use of PKA-mediated lipolytic products to repress futile autophagic degradation during short-term fasting through AMPK inhibition. |
| Institute: | Seoul National University |
| Last Name: | Ji |
| First Name: | Yul |
| Address: | San 56-1, Sillim-Dong, Kwanak-Gu, Seoul, Seoul, 08826, Korea, South |
| Email: | withyul@snu.ac.kr |
| Phone: | 01025076912 |
Summary of all studies in project PR001409
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002206 | Lipolysis-derived Lipids Determine Autophagy Initiation during Fasting | Caenorhabditis elegans | Seoul National University | MS | 2022-07-22 | 1 | 9 | Uploaded data (831K) |
