Summary of project PR001420
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001420. The data can be accessed directly via it's Project DOI: 10.21228/M85M5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001420 |
| Project DOI: | doi: 10.21228/M85M5J |
| Project Title: | Metabolomics of bone marrow-derived dendritic cells conditioned with H. polygyrus bakery non-polar metabolites |
| Project Summary: | Aim: Characterize tolerogenic responses induced by helminth-derived metabolites (HDM) in dendritic cells (DCs). Methods: H. polygyrus worms were culture for 24h and HDMs were isolated from conditioned media by chromatography. Bone marrow-derived dendritic cells (BMDCs) were treated with HDM for 4 or 20 h. Cells were either stimulated with LPS or adoptively transferred to mice. Cytokine secretion was measured by ELISA. The metabolome of HDM-treated DCs were assessed by mass spectrometry, respectively. Results: Pre-treatment with HDM decreased LPS-induced TNF and increased IL-10 release by BMDCs. Importantly, HDM decreased expression of MHC-II, CD86, and CD40 in BMDCs and splenic DCs, suggesting that HDM induces a tolerogenic profile on DCs. The metabolomic approach revealed a total of 17 downregulated metabolites, against one upregulated of the 225 total peaks analyzed. Functional analyses were performed and results predicted a total of 29 pathways and 43 matched compounds. Scatter plot test of significant peaks revealed two differentially enriched pathways, the sphingolipid metabolism, and a highly enriched pathway, the terpenoid backbone metabolism, witch C00418 metabolite is a potential match to mevalonic acid, according to KEGG compound database in HDM-treated DCs in comparison with naïve DCs. These differentially expressed genes and enriched metabolites may indicate a novel mechanism by which helminths induce a tolerogenic profile in DCs. |
| Institute: | McGill University |
| Last Name: | Lopes |
| First Name: | Fernando |
| Address: | 21111 Lakeshore Rd |
| Email: | fernando.lopes@mcgill.ca |
| Phone: | 5143987607 |
Summary of all studies in project PR001420
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002230 | Metabolomics of bone marrow-derived dendritic cells conditioned with H. polygyrus bakery non-polar metabolites | Mus musculus | McGill University | MS* | 2022-07-28 | 1 | 18 | Uploaded data (1.6G)* |
